Sun Yingxin, Wang Beier, Zhang Lichao, Zheng Xiaohan, Xu Peng, Zhang Meng, Han Meiguang, Di Peng, Han Mei, Cheng Lin, Yang Limin
Cultivation Base of State Key Laboratory for Ecological Restoration and Ecosystem Management, College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, China.
Technology Service Center on Ecological Planting of Chinese Herbal Medicine in Chengde, Chengde, China.
Front Plant Sci. 2025 Jan 27;15:1506805. doi: 10.3389/fpls.2024.1506805. eCollection 2024.
The bHLH gene family plays a critical role in regulating internal responses in plants. Although the pharmacological properties of have been extensively studied, its bHLH gene family remains poorly investigated. In this study, 142 genes were identified using the complete genome data of . Phylogenetic and conserved motif analyses were performed. Gene duplication events were analyzed, and cis-element analysis was conducted to explore regulatory factors. The expression patterns of these genes in different tissues and under drought stress were investigated using transcriptome data and qRT-PCR analysis. Phylogenetic and conserved motif analyses revealed that the gene structures within each clade are relatively conserved. Gene duplication analysis identified 29 duplication events in the SbbHLH gene family, most of which involved gene pairs under purifying selection. Cis-element analysis revealed that these genes are regulated by various environmental and hormonal factors. Transcriptomic data and qRT-PCR results demonstrated tissue-specific expression patterns for the 142 genes. Additionally, genes potentially involved in baicalin biosynthesis were identified under drought stress. The findings suggest that under drought stress, , and are regulated by a network centered on , which enhances baicalin biosynthesis. In conclusion, this study provides a comprehensive analysis of the bHLH gene family in and identifies 4 potential genes involved in regulating baicalin biosynthesis under drought stress.
bHLH基因家族在调节植物内部反应中起着关键作用。尽管[植物名称]的药理特性已得到广泛研究，但其bHLH基因家族仍研究较少。在本研究中，利用[植物名称]的全基因组数据鉴定出142个bHLH基因。进行了系统发育和保守基序分析。分析了基因复制事件，并进行顺式元件分析以探索调控因子。利用转录组数据和qRT-PCR分析研究了这些基因在不同组织和干旱胁迫下的表达模式。系统发育和保守基序分析表明，每个[植物名称]进化枝内的基因结构相对保守。基因复制分析在SbbHLH基因家族中鉴定出29个复制事件，其中大多数涉及纯化选择下的基因对。顺式元件分析表明，这些基因受多种环境和激素因子调控。转录组数据和qRT-PCR结果显示142个[植物名称]基因具有组织特异性表达模式。此外，在干旱胁迫下鉴定出可能参与黄芩苷生物合成的基因。研究结果表明，在干旱胁迫下，[植物名称]、[植物名称]和[植物名称]受以[植物名称]为中心的网络调控，该网络增强了黄芩苷的生物合成。总之，本研究对[植物名称]的bHLH基因家族进行了全面分析，并鉴定出4个在干旱胁迫下参与调控黄芩苷生物合成的潜在[植物名称]基因。
