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[转录起始：离子强度和放线菌素D对大肠杆菌RNA聚合酶与T7 DNA之间开放启动子复合物形成动力学的影响]

[Transcription initiation: influence of ionic strength and actinomycin D on the kinetics of the open promoter complex formation between Escherichia coli RNA-polymerase and T7 DNA].

作者信息

Zavriev S K, Belintsev B N, Shemiakin M F

出版信息

Mol Biol (Mosk). 1979 Sep-Oct;13(5):1077-84.

PMID:388191
Abstract

A membrane filter assay has been devised to study the influence of ionic strength (0--150 mM NaCl) and actinomycin D on the kinetics of the open promoter complex formation between E. coli RNA-polymerase and [3H]DNA of T7 phage. The dependence of the complex formation rate upon the ionic strength is non-monotoneus with a maximum at 75--100 mM. The addition of one actinomycin molecule per 200 base pairs decreases the open promoter complex formation rate at ionic strength less than 100 mM. A promoter site selection model including liner diffusional selection is proposed which is in a good agreement with the experimental results obtained.

摘要

已设计出一种膜过滤测定法，用于研究离子强度（0 - 150 mM NaCl）和放线菌素D对大肠杆菌RNA聚合酶与T7噬菌体[3H]DNA之间开放启动子复合物形成动力学的影响。复合物形成速率对离子强度的依赖性是非单调的，在75 - 100 mM时达到最大值。每200个碱基对添加一个放线菌素分子会降低离子强度小于100 mM时开放启动子复合物的形成速率。提出了一个包括线性扩散选择的启动子位点选择模型，该模型与所获得的实验结果高度吻合。

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[Transcription initiation: influence of ionic strength and actinomycin D on the kinetics of the open promoter complex formation between Escherichia coli RNA-polymerase and T7 DNA].[转录起始：离子强度和放线菌素D对大肠杆菌RNA聚合酶与T7 DNA之间开放启动子复合物形成动力学的影响]
Mol Biol (Mosk). 1979 Sep-Oct;13(5):1077-84.
2
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引用本文的文献

1
RNA polymerase-dependent mechanism for the stepwise T7 phage DNA transport from the virion into E. coli.RNA聚合酶依赖的机制，用于T7噬菌体DNA从病毒体逐步转运到大肠杆菌中。
Nucleic Acids Res. 1982 Mar 11;10(5):1635-52. doi: 10.1093/nar/10.5.1635.
2
On the promoter complex formation rate of E. coli RNA polymerases with T7 phage DNA.关于大肠杆菌RNA聚合酶与T7噬菌体DNA的启动子复合物形成速率
Nucleic Acids Res. 1980 Mar 25;8(6):1391-404. doi: 10.1093/nar/8.6.1391.