Zavriev S K, Belintsev B N, Shemiakin M F
Mol Biol (Mosk). 1982 Jan-Feb;16(1):156-9.
The kinetics of promoter complex formation of E. coli RNA polymerase with T7 phage DNA was studied under different ionic strengths at DNA concentrations 0.15, 1.5 and 20 microns/ml. Based on the results obtained the non-specific binding constants, Keq, and the promoter complex formation rate constants, kp, were estimated. Within ionic strength range 0 + 125 mM NaCl the Keq and kp vary from 5.10(6) to 9.10(5) M-1 and from 0.8 x 0.26 x 10(9) M-1 S-1, respectively. The results are discussed from the view point of the one-dimensional diffusion sliding of the enzyme along DNA in the process of phomoter site selection.
在DNA浓度为0.15、1.5和20微克/毫升的情况下，于不同离子强度下研究了大肠杆菌RNA聚合酶与T7噬菌体DNA形成启动子复合物的动力学。根据所得结果估算了非特异性结合常数Keq和启动子复合物形成速率常数kp。在0至125 mM NaCl的离子强度范围内，Keq和kp分别在5×10⁶至9×10⁵ M⁻¹以及0.8至0.26×10⁹ M⁻¹ s⁻¹之间变化。从启动子位点选择过程中酶沿DNA的一维扩散滑动的角度对结果进行了讨论。
