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1
On the promoter complex formation rate of E. coli RNA polymerases with T7 phage DNA.关于大肠杆菌RNA聚合酶与T7噬菌体DNA的启动子复合物形成速率
Nucleic Acids Res. 1980 Mar 25;8(6):1391-404. doi: 10.1093/nar/8.6.1391.
2
[Transcription initiation: influence of ionic strength and actinomycin D on the kinetics of the open promoter complex formation between Escherichia coli RNA-polymerase and T7 DNA].[转录起始：离子强度和放线菌素D对大肠杆菌RNA聚合酶与T7 DNA之间开放启动子复合物形成动力学的影响]
Mol Biol (Mosk). 1979 Sep-Oct;13(5):1077-84.
3
Binding of Escherichia coli RNA polymerase holoenzyme to bacteriophage T7 DNA. Measurements of the rate of open complex formation at T7 promoter A.大肠杆菌RNA聚合酶全酶与噬菌体T7 DNA的结合。T7启动子A处开放复合物形成速率的测定。
J Mol Biol. 1982 Feb 15;155(1):31-51. doi: 10.1016/0022-2836(82)90490-9.
4
[Kinetic determination of the non-specific constants for the binding of Escherichia coli RNA polymerase to T7 phage DNA].[大肠杆菌RNA聚合酶与T7噬菌体DNA结合的非特异性常数的动力学测定]
Mol Biol (Mosk). 1982 Jan-Feb;16(1):156-9.
5
Binding of Escherichia coli RNA polymerase holoenzyme to bacteriophage T7 DNA. Measurements of binding at bacteriophage T7 promoter A1 using a template competition assay.大肠杆菌RNA聚合酶全酶与噬菌体T7 DNA的结合。使用模板竞争分析法对噬菌体T7启动子A1处的结合进行测量。
J Mol Biol. 1982 Feb 15;155(1):1-29. doi: 10.1016/0022-2836(82)90489-2.
6
Bacteriophage T7 E promoter: identification and measurement of kinetics of association with Escherichia coli RNA polymerase.噬菌体T7 E启动子：与大肠杆菌RNA聚合酶结合动力学的鉴定与测定
Biochemistry. 1985 Apr 23;24(9):2219-27. doi: 10.1021/bi00330a016.
7
Gene 2 protein of bacteriophage T7: purification and requirement for packaging of T7 DNA in vitro.噬菌体T7的基因2蛋白：体外纯化及T7 DNA包装的需求
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4852-6. doi: 10.1073/pnas.76.10.4852.
8
Binding of Escherichia coli ribonucleic acid polymerase holoenzyme to a bacteriophage T7 promoter-containing fragment: evaluation of promoter binding constants as a function of solution conditions.大肠杆菌核糖核酸聚合酶全酶与含噬菌体T7启动子片段的结合：作为溶液条件函数的启动子结合常数评估
Biochemistry. 1980 Jul 22;19(15):3504-15. doi: 10.1021/bi00556a015.
9
[The effect of mutation in the beta-subunit of Escherichia coli RNA polymerase on the selectivity of complex formation with T7 DNA promoters].[大肠杆菌RNA聚合酶β亚基突变对与T7 DNA启动子形成复合物的选择性的影响]
Dokl Akad Nauk SSSR. 1989;309(2):487-91.
10
Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.大肠杆菌RNA聚合酶与噬菌体T7早期启动子之间的相互作用。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):122-6. doi: 10.1073/pnas.77.1.122.

引用本文的文献

1
Single-molecule studies of RNA polymerase: motoring along.RNA聚合酶的单分子研究：不断前行
Annu Rev Biochem. 2008;77:149-76. doi: 10.1146/annurev.biochem.77.073106.100741.
2
Facilitated diffusion of a DNA binding protein on chromatin.一种DNA结合蛋白在染色质上的易化扩散。
EMBO J. 1986 Dec 1;5(12):3313-9. doi: 10.1002/j.1460-2075.1986.tb04645.x.

本文引用的文献

1
The 5'-terminal nucleotides of T7 bacteriophage deoxyribonucleic acid.T7噬菌体脱氧核糖核酸的5'-末端核苷酸
J Mol Biol. 1966 Jan;15(1):49-61. doi: 10.1016/s0022-2836(66)80208-5.
2
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.一种来自流感嗜血杆菌的限制性内切酶。I. 纯化及一般特性。
J Mol Biol. 1970 Jul 28;51(2):379-91. doi: 10.1016/0022-2836(70)90149-x.
3
Electron microscopic localization of the binding sites of Escherichia coli RNA polymerase in the early promoter region of T7 DNA.大肠杆菌RNA聚合酶结合位点在T7 DNA早期启动子区域的电子显微镜定位
Eur J Biochem. 1974 May 15;44(2):617-24. doi: 10.1111/j.1432-1033.1974.tb03519.x.
4
Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. II. The effect of alterations in ionic strength of chain initiation and on the conformation of binary complexes.大肠杆菌核糖核酸聚合酶与T7脱氧核糖核酸结合时核糖核酸链起始的研究。II. 离子强度变化对链起始及二元复合物构象的影响。
J Biol Chem. 1974 May 25;249(10):3002-6.
5
Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. I. An assay for the rate and extent of ribonucleic acid chain initiation.大肠杆菌核糖核酸聚合酶与T7脱氧核糖核酸结合时核糖核酸链起始的研究。I. 核糖核酸链起始速率和程度的测定方法
J Biol Chem. 1974 May 25;249(10):2995-3001.
6
RNA polymerase-DNA complexes. 3. Binding of actinomycin D to RNA polymerase-DNA complex.RNA聚合酶-DNA复合物。3.放线菌素D与RNA聚合酶-DNA复合物的结合。
Biochim Biophys Acta. 1973 Feb 4;294(1):425-33.
7
Studies of the binding of Escherichia coli RNA polymerase to DNA. II. The kinetics of the binding reaction.大肠杆菌RNA聚合酶与DNA结合的研究。II. 结合反应的动力学
J Mol Biol. 1972 Sep 28;70(2):187-95. doi: 10.1016/0022-2836(72)90532-3.
8
Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection.大肠杆菌RNA聚合酶与DNA结合的研究。I. σ亚基在位点选择中的作用。
J Mol Biol. 1972 Sep 28;70(2):157-85. doi: 10.1016/0022-2836(72)90531-1.
9
An adenosine triphosphate-dependent deoxyribonuclease from Hemophilus influenzae Rd. I. Purification and properties of the enzyme.来自流感嗜血杆菌Rd的一种依赖三磷酸腺苷的脱氧核糖核酸酶。I. 酶的纯化及性质
J Biol Chem. 1972 May 10;247(9):2846-53.
10
Diffusion controlled reaction rates in spheroidal geometry. Application to repressor--operator association and membrane bound enzymes.球状几何形状中的扩散控制反应速率。在阻遏物 - 操纵基因缔合及膜结合酶中的应用。
Biophys Chem. 1974 Oct;2(3):255-63. doi: 10.1016/0301-4622(74)80050-5.

关于大肠杆菌RNA聚合酶与T7噬菌体DNA的启动子复合物形成速率

On the promoter complex formation rate of E. coli RNA polymerases with T7 phage DNA.

作者信息

Belintsev B N, Zavriev S K, Shemyakin M F

出版信息

Nucleic Acids Res. 1980 Mar 25;8(6):1391-404. doi: 10.1093/nar/8.6.1391.

DOI:10.1093/nar/8.6.1391
PMID:7001366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC323999/
Abstract

Influence of ionic strength on the kinetics of the promoter complex formation between E. coli RNA polymerase and T7 phage DNA was investigated using a membrane filter assay. The enzyme-promoter association rate constant was determined. It varies from 10(9) to 3 x 10(7) M-1 sec-1 when the ionic strength is changed from zero to 0.15 M NaCl. Basing on the theoretical analysis of experimental data obtained the model for the promoter site selection assuming the enzyme sliding along the DNA is discussed.

摘要

使用膜滤器测定法研究了离子强度对大肠杆菌RNA聚合酶与T7噬菌体DNA之间启动子复合物形成动力学的影响。测定了酶与启动子的缔合速率常数。当离子强度从零变化到0.15 M NaCl时，其值从10⁹到3×10⁷ M⁻¹ s⁻¹不等。基于对所得实验数据的理论分析，讨论了假设酶沿DNA滑动的启动子位点选择模型。