Blood Center of Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil; Medical School of Ribeirão Preto, Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
Butantan Institute, São Paulo, Brazil.
J Infect Public Health. 2024 Jul;17(7):102442. doi: 10.1016/j.jiph.2024.04.026. Epub 2024 May 15.
We aimed to describe the landscape, including molecular, epidemiological, and clinical aspects of CHIKV infections in the Ribeirao Preto region, an area endemic to dengue. We randomly screened 3744 plasma samples that had undergone DENV diagnosis to evaluate CHIKV-RNA using an in-house RT-PCR assay. Positive samples were followed clinically, and RNA samples were submitted to whole genome sequencing. Seventeen cases (0.5 %) were positive for CHIKV-RNA despite being negative for DENV-RNA. Notably, half of the patients experienced prolonged arthralgia lasting more than 90 days. Compared with the healthy control group, leukopenia and thrombocytopenia were observed in all CHIKV-positive individuals with statistically significant P values (P < 0.0001 and P = 0.0003, respectively). The genomic analysis revealed that the CHIKV strains being studied are classified within the East-Central-South-African (ECSA) genotype. This analysis identified new mutations, E1: K211E and E2: V264A, while the previously known mutation E1: A226V was not detected among these strains. This study highlights the need for epidemiological surveillance and preparedness for potential CHIKV epidemics in Brazil, particularly where other arboviruses co-circulate.
我们旨在描述里贝朗普雷图地区（登革热流行地区）基孔肯雅热病毒感染的情况，包括分子、流行病学和临床方面。我们随机筛选了 3744 份已进行登革热病毒诊断的血浆样本，使用内部 RT-PCR 检测法评估基孔肯雅热病毒 RNA。对阳性样本进行临床随访，并将 RNA 样本提交进行全基因组测序。尽管 DEN 病毒 RNA 检测结果为阴性，但仍有 17 例（0.5%）样本基孔肯雅热病毒 RNA 检测结果为阳性。值得注意的是，有一半的患者经历了持续 90 天以上的关节痛。与健康对照组相比，所有基孔肯雅热病毒阳性个体均出现白细胞减少症和血小板减少症，具有统计学显著意义（P 值分别为<0.0001 和 P=0.0003）。基因组分析表明，所研究的基孔肯雅热病毒株属于东中非-南非（ECSA）基因型。该分析确定了新的突变，E1：K211E 和 E2：V264A，而之前已知的突变 E1：A226V 在这些株系中未被检测到。本研究强调了在巴西，特别是在其他虫媒病毒共同传播的地区，需要进行流行病学监测和准备，以应对潜在的基孔肯雅热病毒流行。
