外源激素处理下桑椹果实（红果 2 号）中黄酮类生物合成机制的代谢组学和转录组学分析。

Metabolomic and transcriptomic analysis of flavonoids biosynthesis mechanisms in mulberry fruit (Hongguo 2) under exogenous hormone treatments.

机构信息

The Sericultural and Silk Research Institute, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China; Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.

The Sericultural and Silk Research Institute, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, China.

出版信息

Plant Physiol Biochem. 2024 Jul;212:108773. doi: 10.1016/j.plaphy.2024.108773. Epub 2024 May 25.

DOI:10.1016/j.plaphy.2024.108773

PMID:38820912

Abstract

The mulberry fruit is prized for its superior nutrition value and abundant color due to its high flavone content. To enhance comprehension of flavone biogenesis induced by external hormones, we sprayed exogenous ethylene (ETH), indoleacetic acid (IAA) and spermine (SPM) on mulberry fruit (Hongguo 2) during its color-changed period. The levels of anthocyanin, titratable acid, soluble sugar and endogenous hormones were determined after hormone treatment, integrated transcriptome and metabolome analysis were performed for mechanism exploration. Our results indicated that exogenous ETH, SPM, and IAA play important roles in mulberry ripening, including acid reduction, sugar increase and flavonoid synthesis.

摘要

桑树果实因其高黄酮含量而具有卓越的营养价值和丰富的色泽。为了增强对外源激素诱导黄酮生物合成的理解，我们在桑树果实（红果 2 号）变色期喷施外源乙烯（ETH）、吲哚乙酸（IAA）和亚精胺（SPM）。激素处理后测定了花青素、滴定酸、可溶性糖和内源激素的水平，并进行了整合转录组和代谢组分析以探索机制。结果表明，外源 ETH、SPM 和 IAA 在桑树成熟过程中发挥重要作用，包括降低酸度、增加糖分和合成类黄酮。