College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Int J Mol Sci. 2020 Mar 5;21(5):1789. doi: 10.3390/ijms21051789.
Mulberry sclerotiniose caused by is a devastating disease of mulberry ( L.) fruit in Northwest China. At present, no disease-resistant varieties are used in production, as the molecular mechanisms of this disease are not well understood. In this study, to explore new prevention methods and provide direction for molecular breeding, transcriptomic sequencing and un-targeted metabolomics were performed on healthy (CK), early-stage diseased (HB1), and middle-stage diseased (HB2) mulberry fruits. Functional annotation, gene ontology, a Kyoto encyclopedia of genes and genomes (KEGG) analysis, and a Mapman analysis of the differentially expressed genes revealed differential regulation of genes related to plant hormone signal transduction, transcription factors, and phenylpropanoid biosynthesis. A correspondence between the transcript pattern and metabolite profile was observed in the phenylpropanoid biosynthesis pathway. It should be noted that the log2 ratio of eugenol (isoeugenol) in HB1 and HB2 are 85 times and 23 times higher than CK, respectively. Our study shows that phenylpropanoid biosynthesis may play an essential role in response to sclerotiniose pathogen infection and eugenol(isoeugenol) enrichment in mulberry fruit, which may provide a novel method for mulberry sclerotiniose control.
由引起的桑疫病是中国西北地区桑树（L.）果实的毁灭性病害。目前，生产中未使用抗病品种，因为该病的分子机制尚不清楚。在这项研究中，为了探索新的防治方法，并为分子育种提供方向，对健康（CK）、早期发病（HB1）和中期发病（HB2）桑果进行了转录组测序和非靶向代谢组学分析。差异表达基因的功能注释、基因本体论、京都基因与基因组百科全书（KEGG）分析和 Mapman 分析表明，与植物激素信号转导、转录因子和苯丙烷生物合成相关的基因受到差异调控。在苯丙烷生物合成途径中观察到转录模式和代谢谱之间的对应关系。值得注意的是，HB1 和 HB2 中丁香酚（异丁香酚）的 log2 比值分别比 CK 高 85 倍和 23 倍。我们的研究表明，苯丙烷生物合成可能在响应炭疽病菌感染和桑果中丁香酚（异丁香酚）富集中发挥重要作用，这可能为桑树炭疽病的防治提供新方法。
