Armstrong-Major J, Champney W S
Biochim Biophys Acta. 1985 Feb 20;824(2):140-5. doi: 10.1016/0167-4781(85)90090-9.
A comparison was made of bacteriophage MS2 RNA translation in infected Escherichia coli cells and in a defined cell-free system. A number of temperature-sensitive mutants were used as hosts for viral RNA translation at permissive and restrictive temperatures. The amount of viral coat protein synthesis was determined after gel electrophoresis of proteins from the cell lysates. These results were compared to those obtained with cell-free translation assays conducted with ribosomes isolated from the same mutants. Compared with control cells, a reduced activity in vivo and in vitro was found for each mutant examined at elevated temperatures. A good correlation between the two types of translational assays was observed. These findings are discussed in terms of the translational defects known to be a characteristic of some of these mutant strains.
对感染的大肠杆菌细胞和特定无细胞系统中噬菌体MS2 RNA的翻译进行了比较。使用了一些温度敏感突变体作为宿主，在允许温度和限制温度下进行病毒RNA翻译。通过对细胞裂解物中的蛋白质进行凝胶电泳，确定病毒衣壳蛋白的合成量。将这些结果与使用从相同突变体分离的核糖体进行的无细胞翻译测定结果进行比较。与对照细胞相比，在高温下检测的每个突变体在体内和体外的活性均降低。观察到两种翻译测定之间具有良好的相关性。根据已知是其中一些突变菌株特征的翻译缺陷对这些发现进行了讨论。
