Yates J L, Gette W R, Furth M E, Nomura M
Proc Natl Acad Sci U S A. 1977 Feb;74(2):689-93. doi: 10.1073/pnas.74.2.689.
In a DNA-dependent protein-synthesizing system that contains streptomycin-sensitive ribosomes, lambda DNA directs the synthesis of two proteins that are products of the O gene. The larger is produced as a result of read-through of a UGA termination codon. In the system containing streptomycin-resistant ribosomes this read-through protein is not synthesized, indicating that the mutational alteration in the ribosomal protein S12 restricts the read-through. The mutant ribosomes also fail to synthesize the read-through coat protein of RNA phage Qbeta. In addition, the mutant ribosomes restrict suppression of amber mutations in vitro, similar to their effect in vivo.
在一个含有对链霉素敏感核糖体的依赖DNA的蛋白质合成系统中，λ噬菌体DNA指导合成两种蛋白质，它们是O基因的产物。较大的蛋白质是由于UGA终止密码子通读而产生的。在含有对链霉素抗性核糖体的系统中，这种通读蛋白不被合成，这表明核糖体蛋白S12中的突变改变限制了通读。突变核糖体也不能合成RNA噬菌体Qβ的通读外壳蛋白。此外，突变核糖体在体外限制琥珀突变的抑制作用，类似于它们在体内的作用。
