Gheorghiu M, Mouton D, Lecoeur H, Lagranderie M, Mevel J C, Biozzi G
Clin Exp Immunol. 1985 Jan;59(1):177-84.
The resistance to Mycobacterium bovis (BCG) of lines of mice selected for high (H) or low (L) antibody responsiveness was estimated from the rate of BCG multiplication in the organs. During the first 2 weeks after i.v. infection with 5 X 10(6) CFU, BCG multiplied faster in the spleens of H than of L mice. Afterwards there was a more drastic reduction of viable BCG counts in H mice than in L mice so that the residual BCG counts were significantly lower in H mice than in L mice, not only in the spleen but also in the liver and lungs. On the 14th day of infection, the spleen and liver enlargement and the increase of phagocytic activity were similar in the two lines, suggesting an identical T lymphokine release. In contrast with BCG, during the first 2 weeks after infection with 7 X 10(5) CFU, M. tuberculosis (H37Rv) multiplied in the spleens of L mice at a similar or a slightly faster rate than in the spleens of H mice. On the 4th week, the viable H37Rv counts were reduced in H mice whereas L mice did not survive the infection. In mice vaccinated with BCG 5 months before virulent challenge, the multiplication of H37Rv was inhibited in the H and L lines. The protective effect of BCG is therefore stronger in L mice taking into account their higher innate susceptibility to H37Rv. This might be due to the higher level of living BCG found in L mice at the time of challenge.
通过测量卡介苗在器官中的繁殖速率，评估了因高（H）或低（L）抗体反应性而被选择的品系小鼠对牛分枝杆菌（卡介苗）的抵抗力。在静脉注射5×10⁶CFU感染后的前2周内，卡介苗在H品系小鼠脾脏中的繁殖速度比L品系小鼠更快。此后，H品系小鼠体内存活的卡介苗数量比L品系小鼠减少得更为剧烈，以至于不仅在脾脏中，而且在肝脏和肺脏中，H品系小鼠的残留卡介苗数量均显著低于L品系小鼠。在感染后的第14天，两个品系小鼠的脾脏和肝脏肿大以及吞噬活性的增加情况相似，这表明T淋巴细胞因子的释放情况相同。与卡介苗不同，在感染7×10⁵CFU后的前2周内，结核分枝杆菌（H37Rv）在L品系小鼠脾脏中的繁殖速度与在H品系小鼠脾脏中的繁殖速度相似或略快。在第4周时，H品系小鼠体内存活的H37Rv数量减少，而L品系小鼠在感染中未能存活。在对强毒攻击前5个月接种卡介苗的小鼠中，H37Rv在H和L品系小鼠中的繁殖均受到抑制。因此，考虑到L品系小鼠对H37Rv的天然易感性较高，卡介苗对L品系小鼠的保护作用更强。这可能是由于在攻击时L品系小鼠体内存在更高水平的活卡介苗。
