Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plant, Ministry of Education/ College of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China.
Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plant, Ministry of Education/ College of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China.
Int J Biol Macromol. 2024 Jun;272(Pt 2):132703. doi: 10.1016/j.ijbiomac.2024.132703. Epub 2024 May 30.
Lead ion is very harmful to the environment, so it is very important to study its detection methods. In this study, a novel electrochemical sensor was constructed by modifying deoxyribonucleic acid (DNA) on the electrode, which can be used for the detection of Pb in the environment. Part of the mixed solution of chitosan (CS) and Pb template ions was dropped onto the surface of a glassy carbon electrode. CS-Pb film was cross-linked through sodium tripolyphosphate. And a novel DNA-imprinted sensor was prepared by electrodepositing CS-Pb thin film with gold nanoparticles (AuNPs), removing Pb templates, and immobilizing specific double-stranded DNA. The electroactive area, surface morphology, sensitivity, and electrochemical reaction mechanism of the DNA-imprinted sensor were analyzed. The elementary reaction steps were studied through electrochemical reaction kinetics analysis. The experimental results indicate that the DNA-imprinted electrochemical biosensor can quantitatively detect Pb in the range of 10-100 μM (R = 0.9935), and its detection limit is 6.5074 μM (3σ/slope). The sensitivity of the electrochemical biosensor is 1.55233 × 10 A/μM, and its active areas is 6.233 cm. The desorption mechanism and adsorption mechanism have been explored through dynamic parameter analysis. The novel DNA imprinted electrochemical biosensor developed in this paper provides a robust method for detecting lead ions in solution. Additionally, it establishes a solid groundwork for detecting other metal ions.
铅离子对环境非常有害，因此研究其检测方法非常重要。在这项研究中，通过在电极上修饰脱氧核糖核酸（DNA）构建了一种新型电化学传感器，可用于检测环境中的 Pb。将壳聚糖（CS）和 Pb 模板离子的混合溶液的一部分滴在玻碳电极的表面。通过三聚磷酸钠交联 CS-Pb 薄膜。通过在 CS-Pb 薄膜上电沉积金纳米粒子（AuNPs）、去除 Pb 模板并固定特异性双链 DNA 来制备新型 DNA 印迹传感器。分析了 DNA 印迹传感器的电活性面积、表面形态、灵敏度和电化学反应机制。通过电化学反应动力学分析研究了基本反应步骤。实验结果表明，DNA 印迹电化学生物传感器可以在 10-100 μM 的范围内定量检测 Pb（R=0.9935），其检测限为 6.5074 μM（3σ/斜率）。电化学生物传感器的灵敏度为 1.55233×10 A/μM，其活性面积为 6.233 cm。通过动态参数分析探索了脱附和吸附机制。本文开发的新型 DNA 印迹电化学生物传感器为检测溶液中的铅离子提供了一种强大的方法。此外，它为检测其他金属离子奠定了坚实的基础。
