Kiho T, Sobue S, Ukai S
Gifu Pharmaceutical University, Japan.
Carbohydr Res. 1994 Jan 3;251:81-7. doi: 10.1016/0008-6215(94)84277-9.
A glucan (AG-HN1, [alpha]D +24 degrees) and a heteroglycan (AG-HN2, [alpha]D +26 degrees) were isolated from a hot-water extract of the fruiting bodies of Agrocybe cylindracea. The structures were investigated by a combination of chemical and spectroscopic methods. The results indicated that high molecular weight glucan AG-HN1 is primarily a beta-(1-->6)-branched (1-->3)-beta-D-glucan containing small amounts of (1-->4)-linked and (1-->6)-linked glucopyranosyl residues. Low molecular weight heteroglycan AG-HN2 gives galactose, glucose, fucose, and mannose on hydrolysis and appears to be chiefly composed of (1-->6)-linked gluco- and galacto-pyranosyl residues, many of them branched, and various nonreducing terminal residues. AG-HN1 showed a remarkable hypoglycemic activity in both normal and streptozotocin-induced diabetic mice by ip administration, and its activity was higher than that of AG-HN2.
从柱状田头菇子实体的热水提取物中分离出一种葡聚糖（AG-HN1，αD +24°）和一种杂聚糖（AG-HN2，αD +26°）。通过化学和光谱方法相结合对其结构进行了研究。结果表明，高分子量葡聚糖AG-HN1主要是一种β-(1→6)分支的(1→3)-β-D-葡聚糖，含有少量(1→4)连接和(1→6)连接的吡喃葡萄糖基残基。低分子量杂聚糖AG-HN2水解后产生半乳糖、葡萄糖、岩藻糖和甘露糖，似乎主要由(1→6)连接的葡糖和半乳糖吡喃糖基残基组成，其中许多是分支的，还有各种非还原末端残基。AG-HN1通过腹腔注射在正常和链脲佐菌素诱导的糖尿病小鼠中均表现出显著的降血糖活性，且其活性高于AG-HN2。
