Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China; Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia.
Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia.
Carbohydr Polym. 2024 Sep 1;339:122284. doi: 10.1016/j.carbpol.2024.122284. Epub 2024 May 16.
Interactions between human gut microbiota and dietary fibres (DF) are influenced by the complexity and diversity of both individual microbiota and sources of DF. Based on 480 in vitro fermentations, a full factorial experiment was performed with six faecal inocula representing two enterotypes and three DF sources with nanometer, micrometer, and millimeter length-scales (apple pectin, apple cell walls and apple particles) at two concentrations. Increasing DF size reduced substrate disappearance and fermentation rates but not biomass growth. Concentrated DF enhanced butyrate production and lactate cross-feeding. Enterotype differentiated final microbial compositions but not biomass or fermentation metabolite profiles. Individual donor microbiota differences did not influence DF type or concentration effects but were manifested in the promotion of different functional microbes within each population with the capacity to degrade the DF substrates. Overall, consistent effects (independent of donor microbiota variation) of DF type and concentration on kinetics of substrate degradation, microbial biomass production, gas kinetics and metabolite profiles were found, which can form the basis for informed design of DF for desired rates/sites and consequences of gut fermentation. These results add further evidence to the concept that, despite variations between individuals, the human gut microbiota represents a community with conserved emergent properties.
人体肠道微生物群与膳食纤维（DF）之间的相互作用受到个体微生物群和 DF 来源的复杂性和多样性的影响。基于 480 次体外发酵，采用全因子实验设计，用两种 enterotype 和三种纳米、微米和毫米长度尺度的 DF 来源（苹果果胶、苹果细胞壁和苹果颗粒）在两种浓度下对 6 种粪便接种物进行了实验。DF 尺寸的增加降低了基质的消失和发酵速度，但不影响生物量的生长。浓缩 DF 增强了丁酸盐的产生和乳酸的交叉喂养。Enterotype 区分了最终微生物组成，但不区分生物量或发酵代谢物图谱。个体供体微生物群的差异并不影响 DF 类型或浓度的影响，但表现在每个群体中促进不同功能微生物的生长，这些微生物具有降解 DF 底物的能力。总体而言，发现了 DF 类型和浓度对底物降解动力学、微生物生物量产生、气体动力学和代谢物图谱的一致影响（独立于供体微生物群的变化），这可以为设计具有预期速率/部位和肠道发酵后果的 DF 提供依据。这些结果进一步证明了这样一种观点，即尽管个体之间存在差异，但人类肠道微生物群代表了一个具有保守涌现特性的社区。
