Department of Research, Guangxi Medical University Cancer Hospital, No. 71 Hedi Road, Nanning, 530021, China.
Department of Gastrointestinal Surgery, Third Affiliated Hospital of Guangxi Medical University, Nanning, 530031, China.
BMC Cancer. 2024 Jun 1;24(1):671. doi: 10.1186/s12885-024-12458-5.
The role of novel circular RNAs (circRNAs) in colorectal cancer (CRC) remains to be determined. This study aimed to identify a novel circRNA involved in CRC pathogenesis, assess its diagnostic value, and construct a regulatory network.
Differential expression analysis was conducted using circRNA datasets to screen for differentially expressed circRNAs. The expression of selected circRNAs was validated in external datasets and clinical samples. Diagnostic value of plasma circRNA levels in CRC was assessed. A competing endogenous RNA (ceRNA) network was constructed for the circRNA using TCGA dataset.
Analysis of datasets revealed that hsa_circ_101303 was significantly overexpressed in CRC tissues compared to normal tissues. The upregulation of hsa_circ_101303 in CRC tissues was further confirmed through the GSE138589 dataset and clinical samples. High expression of hsa_circ_101303 was associated with advanced N stage, M stage, and tumor stage in CRC. Plasma levels of hsa_circ_101303 were markedly elevated in CRC patients and exhibited moderate diagnostic ability for CRC (AUC = 0.738). The host gene of hsa_circ_101303 was also found to be related to the TNM stage of CRC. Nine miRNAs were identified as target miRNAs for hsa_circ_101303, and 27 genes were identified as targets of these miRNAs. Subsequently, a ceRNA network for hsa_circ_101303 was constructed to illustrate the interactions between the nine miRNAs and 27 genes.
The study identifies hsa_circ_101303 as a highly expressed circRNA in CRC, which is associated with the progression of the disease. Plasma levels of hsa_circ_101303 show promising diagnostic potential for CRC. The ceRNA network for hsa_circ_101303 provides valuable insights into the regulatory mechanisms underlying CRC.
新型环状 RNA（circRNA）在结直肠癌（CRC）中的作用仍有待确定。本研究旨在鉴定一种新型环状 RNA，参与 CRC 的发病机制，评估其诊断价值，并构建一个调控网络。
使用环状 RNA 数据集进行差异表达分析，筛选差异表达的环状 RNA。在外部数据集和临床样本中验证选定的环状 RNA 的表达。评估血浆环状 RNA 水平在 CRC 中的诊断价值。使用 TCGA 数据集构建环状 RNA 的竞争性内源性 RNA（ceRNA）网络。
数据集分析表明，hsa_circ_101303 在 CRC 组织中明显过表达，与正常组织相比。hsa_circ_101303 在 CRC 组织中的上调通过 GSE138589 数据集和临床样本进一步证实。hsa_circ_101303 的高表达与 CRC 中晚期 N 期、M 期和肿瘤分期相关。CRC 患者血浆 hsa_circ_101303 水平明显升高，对 CRC 具有中等诊断能力（AUC=0.738）。hsa_circ_101303 的宿主基因也与 CRC 的 TNM 分期有关。鉴定出 9 个 miRNA 作为 hsa_circ_101303 的靶标 miRNA，27 个基因作为这些 miRNA 的靶标。随后，构建了 hsa_circ_101303 的 ceRNA 网络，以说明 9 个 miRNA 和 27 个基因之间的相互作用。
本研究鉴定出 hsa_circ_101303 是 CRC 中高度表达的环状 RNA，与疾病的进展有关。血浆 hsa_circ_101303 水平对 CRC 具有良好的诊断潜力。hsa_circ_101303 的 ceRNA 网络为 CRC 的调控机制提供了有价值的见解。
