Quiniou-Debrie M C, Debray-Sachs M, Dardenne M, Czernichow P, Assan R, Bach J F
Diabetes. 1985 Apr;34(4):373-9. doi: 10.2337/diab.34.4.373.
Peripheral lymphocyte subsets were enumerated, using OKT monoclonal sera, in 56 diabetic (43 adults and 13 children) and 20 control subjects. Concomitantly, anti-islet humoral and cellular immunity was tested in vitro and serum thymulin level was measured. In the newly diagnosed patients (less than 30 days; 18 cases), the percent of OKT4+ and OKT8+ cells was reduced, the OKT8+ depletion being particularly pronounced in children. Tests for cellular immunity were positive in 83% of the newly diagnosed diabetic subjects and anti-islet cytotoxic antibodies were detected in 50%. The serum thymulin level was decreased in 2 children. Later on in the course of the disease, a marked reduction in OKT3+, OKT4+, and OKT8+ cell percentage was observed, the mean OKT4/OKT8 ratio being normal or lower than normal. The percent of antibody-positive sera rose to 64%, while anti-islet cellular immunity was detectable in 54%. When extrapancreatic manifestations of probable autoimmune nature were present, anti-islet cellular immunity was detected in 100% of cases, accompanied by cytotoxic antibodies in 54%.
(1) the magnitude of T-cell depletion and/or imbalance in diabetic subjects depended mainly on the duration of the disease, (2) anti-islet cellular immunity was the anomaly most frequently detectable, and (3) a decrease in serum thymulin level was infrequently detected.
使用OKT单克隆血清对56例糖尿病患者（43名成人和13名儿童）和20名对照受试者的外周淋巴细胞亚群进行计数。同时，体外检测抗胰岛体液免疫和细胞免疫，并测量血清胸腺素水平。在新诊断的患者（病程小于30天；18例）中，OKT4 +和OKT8 +细胞百分比降低，OKT8 +细胞耗竭在儿童中尤为明显。83%的新诊断糖尿病受试者细胞免疫检测呈阳性，50%检测到抗胰岛细胞毒性抗体。2名儿童血清胸腺素水平降低。在疾病后期，观察到OKT3 +、OKT4 +和OKT8 +细胞百分比显著降低，平均OKT4/OKT8比值正常或低于正常。抗体阳性血清百分比升至64%，而54%可检测到抗胰岛细胞免疫。当存在可能具有自身免疫性质的胰腺外表现时，100%的病例检测到抗胰岛细胞免疫，54%伴有细胞毒性抗体。
（1）糖尿病患者T细胞耗竭和/或失衡的程度主要取决于病程，（2）抗胰岛细胞免疫是最常检测到的异常，（3）血清胸腺素水平降低很少见。
