酵母修复缺陷菌株中的减数分裂重组和孢子形成。
Meiotic recombination and sporulation in repair-deficient strains of yeast.
作者信息
Dowling E L, Maloney D H, Fogel S
出版信息
Genetics. 1985 Feb;109(2):283-302. doi: 10.1093/genetics/109.2.283.
A genetic system designed to monitor recombination and sporulation in various repair-deficient yeast strains was constructed. Variously heterozygous at seven or eight sites distributed across the genome, the system facilitated sensitive detection of changes in frequency or pattern of meiotic recombination. Ten rad mutants sensitive primarily to UV-irradiation and without terminal blocks in the sporulation process were studied. Seven were defective in excision repair (rad1, rad2, rad3, rad4, rad10, rad14 and rad16), and three were defective in mutagenic repair (rad5, rad9 and rad18). Individually, each mutant displayed behavior consistent with an orthodox meiosis including a wild-type meiotic recombination profile with respect to gene conversion, PMS and intergenic map distances. Accordingly, we conclude that these mutants are without major effect on meiotic heteroduplex formation or correction. However, certain combinations of excision-defective mutants with rad18 exhibited marked ascosporal inviability. Tetraploids homozygous for rad1 and rad18 produce a large proportion of diploid spores containing a recessive lethal.
构建了一个用于监测各种修复缺陷型酵母菌株中重组和孢子形成的遗传系统。该系统在基因组中分布的七个或八个位点上存在不同程度的杂合性，有助于灵敏检测减数分裂重组频率或模式的变化。研究了十个主要对紫外线辐射敏感且在孢子形成过程中无终末阻滞的rad突变体。其中七个在切除修复方面存在缺陷（rad1、rad2、rad3、rad4、rad10、rad14和rad16），三个在诱变修复方面存在缺陷（rad5、rad9和rad18）。单独来看，每个突变体的行为都与正统减数分裂一致，包括在基因转换、PMS和基因间图谱距离方面具有野生型减数分裂重组谱。因此，我们得出结论，这些突变体对减数分裂异源双链体的形成或校正没有重大影响。然而，切除缺陷型突变体与rad18的某些组合表现出明显的子囊孢子 inviability。rad1和rad18纯合的四倍体产生大量含有隐性致死基因的二倍体孢子。
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