Zsigray R M, Hopper J B, Zukowski K, Chesbro W R
Infect Immun. 1985 Mar;47(3):670-3. doi: 10.1128/iai.47.3.670-673.1985.
Conditional virulent strains of Yersinia pestis were used as recipients of an F-lac plasmid from either Escherichia coli 23.10S or E. coli CSH23. The transconjugants of Y. pestis were calcium independent; however, calcium dependence was restored after the loss of the E. coli plasmid. The plasmid contents of several Y. pestis F-lac clones were compared with those of the parent strains and several lac segregants. Although the 6- and 61-megadalton plasmids were detected in all strains tested, the Vwa plasmid was absent in the F-containing strains. The plasmid was evident, however, in the lac segregants derived from the F-lac clones. Results of Southern transfers indicated that the Vwa plasmid had integrated into the chromosome of Y. pestis strains harboring F-lac.
鼠疫耶尔森氏菌的条件致病菌株被用作来自大肠杆菌23.10S或大肠杆菌CSH23的F - lac质粒的受体。鼠疫耶尔森氏菌的转接合子不依赖钙；然而，在大肠杆菌质粒丢失后，钙依赖性得以恢复。将几个鼠疫耶尔森氏菌F - lac克隆的质粒含量与亲本菌株和几个乳糖分离株的质粒含量进行了比较。尽管在所有测试菌株中都检测到了6兆道尔顿和61兆道尔顿的质粒，但含F的菌株中不存在Vwa质粒。然而，在源自F - lac克隆的乳糖分离株中该质粒很明显。Southern转移结果表明，Vwa质粒已整合到携带F - lac的鼠疫耶尔森氏菌菌株的染色体中。
