Bakour R, Laroche Y, Cornelis G
Plasmid. 1983 Nov;10(3):279-89. doi: 10.1016/0147-619x(83)90042-2.
The 70-kb virulence plasmid, vir, from four Yersinia enterocolitica and one Y. pseudotuberculosis strains are incompatible with IncFI plasmids F'Lac and R386 while they are compatible with plasmids representing nine other incompatibility groups. Hybridization experiments carried out on one of these virulence plasmids showed that it contains the F incompatibility determinant D, incD. This determinant was cloned onto pACYC184 and the recombinant clone expressed incompatibility with F'Lac. We conclude that the incompatibility observed between F or R386 and the 70-kb virulence plasmid of Y. enterocolitica and Y. pseudotuberculosis is mediated by incD. Replication genes (rep) from the same plasmid were cloned independently in Escherichia coli. Rep and incD map on two different BamHI fragments. Surprisingly, the replicon isolated is not sensitive to inc D incompatibility. Apart from incD, vir and F share extremely little homology. In particular, there is no evidence for the presence of an F-like transfer operon on vir.
来自四株小肠结肠炎耶尔森氏菌和一株假结核耶尔森氏菌的70kb毒力质粒vir与IncFI质粒F'Lac和R386不兼容，而与代表其他九个不相容群的质粒兼容。对其中一个毒力质粒进行的杂交实验表明，它含有F不相容决定簇D，即incD。该决定簇被克隆到pACYC184上，重组克隆表现出与F'Lac的不相容性。我们得出结论，F或R386与小肠结肠炎耶尔森氏菌和假结核耶尔森氏菌的70kb毒力质粒之间观察到的不相容性是由incD介导的。来自同一质粒的复制基因（rep）在大肠杆菌中独立克隆。Rep和incD定位于两个不同的BamHI片段上。令人惊讶的是，分离出的复制子对incD不相容性不敏感。除了incD外，vir和F的同源性极低。特别是，没有证据表明vir上存在F样转移操纵子。
