Hendry J H
Int J Radiat Biol Relat Stud Phys Chem Med. 1985 Jan;47(1):3-16. doi: 10.1080/09553008514550021.
A survey of survival curves in the literature, for clonogenic cells (clonogens) in normal tissues, highlights the following features: the sensitivity of some human and dog clonogens apparently is greater than that of their counterparts in mice and sheep, assessed in vitro. However, this should be interpreted with caution because of the possibility of cell selection and the ability to modify sensitivity markedly in some systems by variations in growth conditions; extrapolation numbers are in general higher when assessed in vivo than in vitro. This is due partly to the lack of measurements of repair of potentially-lethal damage using many assays in vitro. This feature increases the extrapolation number when measured using transplantation assays in vivo; epithelial clonogens in vivo demonstrate a remarkable similarity in sensitivity between tissues. The range is similar for clonogens assayed in situ or by transplantation, and this argues against the possibility that a resistant subpopulation may be selected in most assays in situ. It is emphasized from the comparisons that caution must be exercised in extrapolating results, obtained for clonogens assayed in vitro or by transplantation in vivo, to the situation in situ.
对文献中正常组织克隆形成细胞（克隆原）存活曲线的一项调查突出了以下特征：在体外评估时，一些人类和犬类克隆原的敏感性显然高于小鼠和绵羊的同类细胞。然而，由于细胞选择的可能性以及在某些系统中生长条件变化会显著改变敏感性，对此应谨慎解读；一般而言，体内评估时的外推数高于体外评估。部分原因是在许多体外试验中缺乏对潜在致死性损伤修复的测量。当在体内使用移植试验进行测量时，这一特征会增加外推数；体内上皮克隆原在不同组织间显示出显著的敏感性相似性。原位或通过移植检测的克隆原范围相似，这表明在大多数原位检测中不太可能选择出抗性亚群。通过比较强调，在将体外或体内移植检测克隆原所获得的结果外推至原位情况时必须谨慎。
