Xiao Chao-Wu, Carbonel Adriana A, Lima Patricia D A, Hendry Amy, Tsang Benjamin K
Nutrition Research Division, Food Directorate, Health Products and Food Branch, Health Canada, Banting Research Centre, Ottawa, ONK1A 0K9, Canada.
Food and Nutrition Science Program, Department of Chemistry, Carleton University, Ottawa, ONK1S 5B6, Canada.
Br J Nutr. 2024 Jun 3;132(4):1-9. doi: 10.1017/S0007114524001296.
Polycystic ovary syndrome is associated with increased risks for certain metabolic disorders such as insulin resistance, non-alcoholic fatty liver disease and suppressed ovarian follicular development. This study aimed to examine whether soya isoflavones (ISF) mitigate these polycystic ovary syndrome-associated metabolic disorders in a rat model. Weanling Sprague-Dawley female rats were randomly divided into six groups and were treated with either 0 or 83 µg/d dihydrotestosterone (DHT) to induce polycystic ovary syndrome and fed diets containing 0, 0·5, or 1 g ISF/kg diet for 8 weeks. DHT treatment increased food intake, body weight gain ( < 0·001), percentage of primordial follicles (60 % . 50·9 %, < 0·05) and accumulation of lipid droplets in the livers. It also elevated serum total cholesterol, free cholesterol, TAG, NEFA and leptin and hepatic total cholesterol and NEFA. Additionally, DHT treatment reduced the percentage of primary follicles (13·8 % . 30·2 %, < 0·05), ovary weight and length ( < 0·001), as well as insulin sensitivity ( < 0·01) compared with the Control. ISF intake at 1 g/kg reduced body weight gain, serum total cholesterol, free cholesterol, NEFA, leptin and hepatic TAG and DHT-induced insulin resistance ( < 0·01). ISF intake at both levels decreased DHT-induced lipid droplet accumulation in the livers and changes in the percentages of primordial and primary follicles. Dietary soya ISF alleviated DHT-induced body weight gain, insulin resistance and hepatic lipid droplet accumulation, as well as suppressed ovarian follicular development. This suggests that the consumption of soya foods or ISF supplements may be beneficial for individuals with polycystic ovary syndrome, mitigating the associated metabolic disorders such as diabetes and non-alcoholic fatty liver disease.
多囊卵巢综合征与某些代谢紊乱风险增加相关，如胰岛素抵抗、非酒精性脂肪肝病和卵巢卵泡发育受抑制。本研究旨在检测大豆异黄酮（ISF）是否能减轻大鼠模型中这些与多囊卵巢综合征相关的代谢紊乱。将断乳的斯普拉格 - 道利雌性大鼠随机分为六组，用0或83μg/d双氢睾酮（DHT）处理以诱导多囊卵巢综合征，并给予含0、0.5或1g ISF/kg饲料的饮食，持续8周。DHT处理增加了食物摄入量、体重增加（<0.001）、原始卵泡百分比（60%对50.9%，<0.05）以及肝脏中脂滴的积累。它还升高了血清总胆固醇、游离胆固醇、甘油三酯、非酯化脂肪酸和瘦素以及肝脏总胆固醇和非酯化脂肪酸。此外，与对照组相比，DHT处理降低了初级卵泡百分比（13.8%对30.2%，<0.05）、卵巢重量和长度（<0.001）以及胰岛素敏感性（<0.01）。1g/kg的ISF摄入量降低了体重增加、血清总胆固醇、游离胆固醇、非酯化脂肪酸、瘦素以及肝脏甘油三酯和DHT诱导的胰岛素抵抗（<0.01）。两个水平的ISF摄入量均减少了DHT诱导的肝脏脂滴积累以及原始卵泡和初级卵泡百分比的变化。膳食大豆ISF减轻了DHT诱导的体重增加、胰岛素抵抗和肝脏脂滴积累，以及抑制了卵巢卵泡发育。这表明食用大豆食品或ISF补充剂可能对多囊卵巢综合征患者有益，可减轻相关的代谢紊乱，如糖尿病和非酒精性脂肪肝病。
