Sachan Rohan Samir Kumar, Devgon Inderpal, Sharma Vikas, Perveen Kahkashan, Bukhari Najat A, Alsulami Jamilah A, Jadon Vikash Singh, Suyal Deep Chandra, Karnwal Arun
School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, 144411, Punjab, India.
Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, 11495, Saudi Arabia.
Heliyon. 2024 May 22;10(11):e31572. doi: 10.1016/j.heliyon.2024.e31572. eCollection 2024 Jun 15.
Wheat is a crucial food crop worldwide, generating straw upon post-harvest. The straw is often burned to enhance soil fertility, leading to massive air pollution. In this study, wheat straw was investigated for the production of Polyhydroxyalkanoate (PHA) using the novel isolate RSKS-3. The wheat straw was pulverized and valorized with different acids (2 % and 4 % HSO, acetic acid, and hydrochloric acid) and alkalis (2 % and 4 % NaOH, calcium carbonate, and potassium hydroxide). The validation of carbohydrates was done using the Molisch test by analyzing purple-ring production and the DNS test which concluded 4 % HSO as an effective treatment with a maximal sugar yield of 5.04 mg/mL at P < 0.05. The bioconversion efficiency of the extract to PHA resulted in 0.87 g/L by RSKS-3, later characterized by Ultraviolet (UV)-spectroscopy and FT-IR assessment. The findings of the research offer a potential strategy to mitigate airborne pollutants that result from smouldering wheat straw, thereby contributing significant improvements to sustainable development.
小麦是全球重要的粮食作物，收获后会产生秸秆。秸秆常被焚烧以提高土壤肥力，导致大量空气污染。在本研究中，利用新分离的菌株RSKS-3研究了小麦秸秆用于生产聚羟基脂肪酸酯（PHA）的情况。将小麦秸秆粉碎，并用不同的酸（2%和4%的硫酸、乙酸和盐酸）和碱（2%和4%的氢氧化钠、碳酸钙和氢氧化钾）进行处理以实现其价值提升。通过莫利施试验分析紫色环的产生以及DNS试验对碳水化合物进行验证，结果得出4%的硫酸是一种有效的处理方法，在P<0.05时最大糖产量为5.04mg/mL。RSKS-3将提取物转化为PHA的生物转化效率为0.87g/L，随后通过紫外光谱和傅里叶变换红外光谱评估对其进行表征。该研究结果提供了一种潜在策略，以减轻小麦秸秆燃烧产生的空气污染物，从而为可持续发展做出重大改进。
