Kurki P, Virtanen I
J Immunol Methods. 1985 Feb 11;76(2):329-35. doi: 10.1016/0022-1759(85)90310-2.
Some human smooth muscle antibodies (SMA) react with cytoskeletal intermediate filament (IMF) antigens. The smooth muscle tissue contains two types of IMF: vimentin and desmin filaments. In this study, SMA of anti-IMF type in 52 patients' sera have been classified into anti-vimentin filament and anti-desmin filament types according to their immunofluorescence staining patterns on rat testis. This classification is based on the fact that the arterial walls of testis contains both vimentin and desmin whereas the myoid cell layer surrounding the seminiferous tubuli contains only desmin. Four out of the 52 sera gave the anti-desmin staining pattern and 40 sera showed the anti-vimentin type of staining. Thirty-two sera were further classified by using cultured human rhabdomyosarcoma (RD) cells as targets. Nine sera reacted with the intermediate filaments of the RD cells. Among these were 3 out of the 4 sera that gave the anti-desmin filament staining pattern. The anti-desmin specificity of SMA was confirmed in 1 serum by the immunoblotting technique. These results indicate that while human anti-desmin filament antibodies exist, most human SMA of anti-IMF type react with vimentin filaments.
一些人平滑肌抗体（SMA）与细胞骨架中间丝（IMF）抗原发生反应。平滑肌组织包含两种类型的中间丝：波形蛋白丝和结蛋白丝。在本研究中，根据52例患者血清中抗IMF型SMA在大鼠睾丸上的免疫荧光染色模式，将其分为抗波形蛋白丝型和抗结蛋白丝型。这种分类基于以下事实：睾丸动脉壁同时含有波形蛋白和结蛋白，而围绕生精小管的肌样细胞层仅含有结蛋白。52份血清中有4份呈现抗结蛋白染色模式，40份血清显示抗波形蛋白染色类型。通过使用培养的人横纹肌肉瘤（RD）细胞作为靶点，对32份血清进行了进一步分类。9份血清与RD细胞的中间丝发生反应。其中，在呈现抗结蛋白丝染色模式的4份血清中有3份。通过免疫印迹技术在1份血清中证实了SMA的抗结蛋白特异性。这些结果表明，虽然存在人抗结蛋白丝抗体，但大多数抗IMF型人SMA与波形蛋白丝发生反应。
