School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Department of Oncology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Nucleic Acids Res. 2024 Jul 8;52(12):7384-7396. doi: 10.1093/nar/gkae470.
The revolutionary technology of CRISPR/Cas has reshaped the landscape of molecular biology and molecular engineering. This tool is of interest to researchers in multiple fields, including molecular diagnostics, molecular biochemistry circuits, and information storage. As CRISPR/Cas spreads to more niche areas, new application scenarios and requirements emerge. Developing programmability and compatibility of CRISPR/Cas becomes a critical issue in the new phase. Here, we report a redundancy-based modular CRISPR/Cas12a synergistic activation platform (MCSAP). The position, length, and concentration of the redundancy in the split DNA activators can finely regulate the activity of Cas12a. With the redundant structure as an interface, MCSAP serves as a modular plug-in to seamlessly integrate with the upstream molecular network. MCSAP successfully performs three different tasks: nucleic acid detection, enzyme detection, and logic operation. MCSAP can work as an effector for different molecular networks because of its compatibility and programmability. Our platform provides powerful yet easy-to-use tools and strategies for the fields of DNA nanotechnology, molecular engineering, and molecular biology.
CRISPR/Cas 的革命性技术改变了分子生物学和分子工程的格局。这一工具引起了包括分子诊断、分子生化电路和信息存储在内的多个领域研究人员的兴趣。随着 CRISPR/Cas 技术扩展到更多的专业领域，新的应用场景和需求不断涌现。开发 CRISPR/Cas 的可编程性和兼容性成为新阶段的关键问题。在这里，我们报告了一种基于冗余的模块化 CRISPR/Cas12a 协同激活平台（MCSAP）。在分割 DNA 激活子中的冗余位置、长度和浓度可以精细调节 Cas12a 的活性。MCSAP 以冗余结构作为接口，作为一个模块化插件，可以与上游分子网络无缝集成。MCSAP 成功执行了三种不同的任务：核酸检测、酶检测和逻辑运算。由于其兼容性和可编程性，MCSAP 可以作为不同分子网络的效应器。我们的平台为 DNA 纳米技术、分子工程和分子生物学领域提供了强大而易用的工具和策略。
