Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Pathology.
Bursa Uludağ University, Faculty of Veterinary Medicine, Department of Pathology.
Vet Ital. 2023 Dec 31;59(4). doi: 10.12834/VetIt.3030.21925.2.
Cells obtained from chicken embryos are often preferred for in vitro studies. These cells, which easily adapt to rapid and continuous growth in the appropriate cell culture environment, are thought to be one of the effective methods in the investigation of leg diseases that are frequently observed in poultry. Leg diseases, especially affecting the joints in chickens, cause locomotor problems and adversely affect animal welfare. In addition, they cause significant health problems and increase mortality. It is known that synovial fibroblasts play an important role in joint diseases. In this study, chicken embryonic synovial fibroblasts were isolated from tissue explants taken from the tibio-metatarsal joint region of brown layer chicken embryos. Characterization of cells was evaluated by immunocytochemistry and hemacolor staining. chicken embryonic synovial fibroblasts showed a strong positive reaction to the vimentin antibody. As a result of hemacolor staining, it was noted that the cell morphology was spindle-shaped. The absence of macrophages in chicken embryonic synovial fibroblast culture was confirmed by the carbon powder uptake. In this present study, we aim to present a useful cell culture protocol such as primary culture, passage, and characterization suitable for chicken embryonic synovial fibroblast to be used in the new scientific research.
从鸡胚中获得的细胞通常是体外研究的首选。这些细胞在适当的细胞培养环境中很容易适应快速和连续的生长，被认为是研究家禽中经常观察到的腿部疾病的有效方法之一。腿部疾病，特别是影响鸡关节的疾病，会导致运动问题，并对动物福利产生不利影响。此外，它们还会引起严重的健康问题并增加死亡率。已知滑膜成纤维细胞在关节疾病中起重要作用。在这项研究中，从褐壳蛋鸡胚胎的胫跗关节区域的组织外植体中分离出鸡胚滑膜成纤维细胞。通过免疫细胞化学和血色素染色评估细胞的特征。鸡胚滑膜成纤维细胞对波形蛋白抗体呈强阳性反应。血色素染色的结果表明，细胞形态呈纺锤形。通过碳粉摄取证实鸡胚滑膜成纤维细胞培养中不存在巨噬细胞。在本研究中，我们旨在提出一种有用的细胞培养方案，如原代培养、传代和鉴定，适合用于新的科学研究的鸡胚滑膜成纤维细胞。
