Dayı Belma, Sevimli Alper, Akkoç Ahmet, Mutlu Ayşe Meriç, Doğan Nurhan
Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.
Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Turkey.
Poult Sci. 2025 Jun 2;104(9):105358. doi: 10.1016/j.psj.2025.105358.
Amyloid arthropathy, which negatively affects animal welfare by causing various health problems in brown laying hens, is a pathological phenomenon that occurs when amyloid A (AA) protein accumulates in the leg joints and serum amyloid A (SAA), the precursor of AA protein, forms permanent amyloid fibrils. This study aimed to evaluate the SAA production at the 24th and 48th h by enzyme-linked immunosorbent antibody assay (ELISA) in culture medium and real-time quantitative polymerase chain reaction (qPCR) after the induction of chicken embryonic synovial fibroblasts (CESF) with different doses of lipopolysaccharide (LPS), interleukin-1β (IL-1β) and vitamin A. Hemacolor staining, immunocytochemistry, and carbon powder uptake test were performed to characterize the isolated CESF in the study. A statistically significant increase was observed in SAA mRNA expression in all groups at the 24th and 48th h, except the group induced with vitamin A for 48 h, while no increase was detected in the SAA level by ELISA. In the qPCR results, the highest increase among the 24-hour groups was 2604.5 ± 476 in the LPS (10 μg/mL) + IL-1β (30 ng/mL) applied group, while the highest increase among the 48-hour groups was 1577.4 ± 326.4 in the LPS (5 μg/mL) + IL-1β (50 ng/mL) + Vitamin A applied group. The results have shown that SAA expression in CESF is particularly dependent on LPS concentration, with IL-1β and vitamin A being less effective CESF from the brown layer hen embryos proved to be a suitable study model for amyloid arthropathy.
淀粉样关节病会给褐壳蛋鸡带来各种健康问题，对动物福利产生负面影响。它是一种病理现象，当淀粉样蛋白A（AA）在腿部关节中积累，且AA蛋白的前体血清淀粉样蛋白A（SAA）形成永久性淀粉样纤维时就会发生。本研究旨在通过酶联免疫吸附抗体测定（ELISA）评估在不同剂量的脂多糖（LPS）、白细胞介素-1β（IL-1β）和维生素A诱导鸡胚滑膜成纤维细胞（CESF）后，在第24小时和第48小时培养基中的SAA产生情况，以及通过实时定量聚合酶链反应（qPCR）进行评估。在该研究中，进行了苏木精染色、免疫细胞化学和碳粉摄取试验以鉴定分离出的CESF。除了用维生素A诱导48小时的组外，在第24小时和第48小时所有组的SAA mRNA表达均有统计学显著增加，而ELISA检测未发现SAA水平升高。在qPCR结果中，24小时组中升高最高的是应用LPS（10μg/mL）+IL-1β（30ng/mL）的组，为2604.5±476，而48小时组中升高最高的是应用LPS（5μg/mL）+IL-1β（50ng/mL）+维生素A的组，为1577.4±326.4。结果表明，CESF中的SAA表达特别依赖于LPS浓度，IL-1β和维生素A的作用较小。褐壳蛋鸡胚胎的CESF被证明是淀粉样关节病的合适研究模型。
