人类皮肤的保存：使用无胸腺（裸）小鼠模型对两种培养基的研究。

Preservation of human skin: a study of two media using the athymic (nude) mouse model.

作者信息

Cram A E, Domayer M A, Scupham R

出版信息

J Trauma. 1985 Feb;25(2):128-30.

Abstract

This study is part of an ongoing attempt to define the optimal conditions for short-term storage of viable human skin for auto- or homotransplantation. Roswell Park Memorial Institute 1640 (RPMI) media was more convenient to use than Eagle's Minimum Essential Medium with HEPES buffer (MEM-H) but functional skin viability was not statistically different in the two media. MEM without HEPES buffer did not preserve human skin at 20 days storage. The athymic (nude) mouse provides an excellent in vivo test of stored human skin functional viability.

摘要

本研究是正在进行的一项尝试的一部分，该尝试旨在确定用于自体或同种异体移植的 viable 人类皮肤短期储存的最佳条件。罗斯威尔帕克纪念研究所 1640（RPMI）培养基比含 HEPES 缓冲液的伊格尔最低必需培养基（MEM-H）使用起来更方便，但两种培养基中功能性皮肤活力在统计学上没有差异。不含 HEPES 缓冲液的 MEM 在储存 20 天时不能保存人类皮肤。无胸腺（裸）小鼠为储存的人类皮肤功能活力提供了出色的体内测试。