Department of Molecular and Integrative Physiology, University of Michigan, BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109, USA.
Geriatrics Center, University of Michigan, Ann Arbor, MI, 48109, USA.
Sci Rep. 2024 Jun 3;14(1):12688. doi: 10.1038/s41598-024-63152-2.
Comprehensive characterization of protein networks in mounted brain tissue represents a major challenge in brain and neurodegenerative disease research. In this study, we develop a simple staining method, called TSWIFT, to iteratively stain pre-mounted formalin fixed, paraffin embedded (FFPE) brain sections, thus enabling high-dimensional sample phenotyping. We show that TSWIFT conserves tissue architecture and allows for relabeling a single mounted FFPE sample more than 10 times, even after prolonged storage at 4 °C. Our results establish TSWIFT as an efficient method to obtain integrated high-dimensional knowledge of cellular proteomes by analyzing mounted FFPE human brain tissue.
在脑组织的固定组织中进行蛋白质网络的全面特征分析是脑科学和神经退行性疾病研究的一个主要挑战。在这项研究中，我们开发了一种简单的染色方法，称为 TSWIFT，用于对预固定、石蜡包埋（FFPE）的脑组织切片进行迭代染色，从而实现高维样本表型分析。我们发现 TSWIFT 能保持组织架构，并允许对单个已固定的 FFPE 样本进行 10 次以上的重新标记，即使在 4°C 下长时间储存后也是如此。我们的结果表明，TSWIFT 是一种通过分析固定的 FFPE 人脑组织获得细胞蛋白质组综合高维知识的有效方法。
