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利用迭代免疫荧光成像技术对衰老齿状回中的神经发生龛进行特征分析。

Characterization of the neurogenic niche in the aging dentate gyrus using iterative immunofluorescence imaging.

机构信息

Brain Research Institute, University of Zurich, Zurich, Switzerland.

Department of Molecular Life Sciences, University of Zurich, Zurich, Switzerland.

出版信息

Elife. 2022 Jan 28;11:e68000. doi: 10.7554/eLife.68000.

DOI:10.7554/eLife.68000
PMID:35089129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8798039/
Abstract

Advancing age causes reduced hippocampal neurogenesis, associated with age-related cognitive decline. The spatial relationship of age-induced alterations in neural stem cells (NSCs) and surrounding cells within the hippocampal niche remains poorly understood due to limitations of antibody-based cellular phenotyping. We established iterative indirect immunofluorescence imaging (4i) in tissue sections, allowing for simultaneous detection of 18 proteins to characterize NSCs and surrounding cells in 2-, 6-, and 12-month-old mice. We show that reorganization of the dentate gyrus (DG) niche already occurs in middle-aged mice, paralleling the decline in neurogenesis. 4i-based tissue analysis of the DG identifies changes in cell-type contributions to the blood-brain barrier and microenvironments surrounding NSCs to play a pivotal role to preserve neurogenic permissiveness. The data provided represent a resource to characterize the principles causing alterations of stem cell-associated plasticity within the aging DG and provide a blueprint to analyze somatic stem cell niches across lifespan in complex tissues.

摘要

随着年龄的增长，海马神经发生减少，与年龄相关的认知能力下降有关。由于基于抗体的细胞表型分析存在局限性，因此对于海马龛内年龄诱导的神经干细胞（NSC）和周围细胞的空间关系仍知之甚少。我们在组织切片中建立了迭代间接免疫荧光成像（4i），允许同时检测 18 种蛋白质，以鉴定 2、6 和 12 个月大的小鼠中的 NSCs 和周围细胞。我们表明，齿状回（DG）龛位的重新组织已经在中年小鼠中发生，与神经发生的下降平行。基于 4i 的 DG 组织分析确定了对血脑屏障和 NSCs 周围微环境的细胞类型贡献的变化，在维持神经发生的许可方面发挥着关键作用。提供的数据代表了一种资源，可以描述导致衰老 DG 中与干细胞相关的可塑性改变的原则，并为分析复杂组织中整个生命周期的体干细胞龛位提供了蓝图。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce9/8798039/dfbfeba9dadb/elife-68000-fig7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2ce9/8798039/dfbfeba9dadb/elife-68000-fig7.jpg
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Nat Rev Neurosci. 2021 Apr;22(4):223-236. doi: 10.1038/s41583-021-00433-z. Epub 2021 Feb 25.
3
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4
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5
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