College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning, 110866, China.
Saint-Petersburg State Agrarian University, Peterburgskoe shosse, Pushkin, St. Petersburg, 196601, Russia.
BMC Plant Biol. 2024 Jun 4;24(1):496. doi: 10.1186/s12870-024-05186-2.
Monosaccharide transporter (MST) family, as a carrier for monosaccharide transport, plays an important role in carbon partitioning and widely involves in plant growth and development, stress response, and signaling transduction. However, little information on the MST family genes is reported in maize (Zea mays), especially in response to abiotic stresses. In this study, the genome-wide identification of MST family genes was performed in maize.
A total of sixty-six putative members of MST gene family were identified and divided into seven subfamilies (including SPT, PMT, VGT, INT, pGlcT, TMT, and ERD) using bioinformatics approaches, and gene information, phylogenetic tree, chromosomal location, gene structure, motif composition, and cis-acting elements were investigated. Eight tandem and twelve segmental duplication events were identified, which played an important role in the expansion of the ZmMST family. Synteny analysis revealed the evolutionary features of MST genes in three gramineous crop species. The expression analysis indicated that most of the PMT, VGT, and ERD subfamilies members responded to osmotic and cadmium stresses, and some of them were regulated by ABA signaling, while only a few members of other subfamilies responded to stresses. In addition, only five genes were induced by NaCl stress in MST family.
These results serve to understand the evolutionary relationships of the ZmMST family genes and supply some insight into the processes of monosaccharide transport and carbon partitioning on the balance between plant growth and development and stress response in maize.
单糖转运蛋白(MST)家族作为单糖转运的载体,在碳分配中发挥着重要作用,广泛参与植物的生长发育、应激响应和信号转导。然而,关于玉米(Zea mays)中 MST 家族基因的信息知之甚少,特别是在应对非生物胁迫方面。本研究在玉米中进行了 MST 家族基因的全基因组鉴定。
利用生物信息学方法,共鉴定出 66 个 MST 基因家族的假定成员,并分为 7 个亚家族(包括 SPT、PMT、VGT、INT、pGlcT、TMT 和 ERD),对基因信息、系统发育树、染色体定位、基因结构、基序组成和顺式作用元件进行了研究。鉴定出 8 个串联和 12 个片段重复事件,这对 ZmMST 家族的扩张起了重要作用。共线性分析揭示了三个禾本科作物物种中 MST 基因的进化特征。表达分析表明,PMT、VGT 和 ERD 亚家族的大多数成员对渗透和镉胁迫有反应,其中一些受 ABA 信号的调控,而其他亚家族的成员只有少数对胁迫有反应。此外,MST 家族中只有 5 个基因对 NaCl 胁迫有诱导作用。
这些结果有助于了解 ZmMST 家族基因的进化关系,并为理解玉米中单糖转运和碳分配过程在植物生长发育和应激响应之间的平衡提供了一些见解。