Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Genes (Basel). 2019 Jan 21;10(1):71. doi: 10.3390/genes10010071.
Sugar transporter protein () genes are involved in multiple biological processes, such as plant responses to various stresses. However, systematic analysis and functional information of family genes in are very limited. A comprehensive analysis was carried out to identify genes and dissect their phylogenetic relationships and to investigate the expression profiles in different organs and in response to the clubroot disease. A total of 22 genes were identified in the genome and they were further classified into four clades based on the phylogenetic analysis. All the BoSTP proteins harbored the conserved sugar transporter (Sugar_tr, PF00083) domain, and the majority of them contained 12 transmembrane helices (TMHs). Rates of synonymous substitution in relative to indicated that genes of diverged from those of approximately 16.3 million years ago. Expression profiles of the genes in different organs derived from RNA-Seq data indicated that a large number of the genes were expressed in specific organs. Additionally, the expression of and genes were induced in roots of the clubroot-susceptible cabbage (CS-JF1) at 28 days after inoculation with , compared with mock-inoculated plants. We speculated that the two BoSTPs might be involved in monosaccharide unloading and carbon partitioning associated with colonization in CS-JF1. Subcellular localization analysis indicated that the two BoSTP proteins were localized in the cell membrane. This study provides insights into the evolution and potential functions of .
糖转运蛋白（Sugar transporter protein，STP）基因参与多种生物过程，如植物对各种胁迫的响应。然而，对 中的 家族基因的系统分析和功能信息非常有限。本研究对 22 个 基因进行了全面分析，鉴定了它们的系统发育关系，并研究了它们在不同器官中的表达谱以及对根肿病的响应。在 基因组中鉴定出 22 个 基因，并根据系统发育分析将其进一步分为四个分支。所有 BoSTP 蛋白都含有保守的糖转运体（Sugar_tr，PF00083）结构域，其中大多数含有 12 个跨膜螺旋（TMHs）。 与 相比，同义替换率表明 基因大约在 1630 万年前从 基因分化而来。来自 RNA-Seq 数据的不同器官中 基因的表达谱表明，大量的 基因在特定器官中表达。此外，在接种 后 28 天，根肿病敏感型白菜（CS-JF1）根中 和 基因的表达被诱导，与 mock 接种的植物相比。我们推测这两个 BoSTP 可能参与了 CS-JF1 中与 定殖相关的单糖卸载和碳分配。亚细胞定位分析表明，这两个 BoSTP 蛋白定位于细胞膜上。本研究为 基因的进化和潜在功能提供了新的认识。
