Wang Jian, Xu Xiuzhi, Li Zhulai, Qiu Bin
Pharmaceutical Chemistry Department, School of Pharmacy, Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, Fujian Medical University, Fuzhou, 350122, PR China.
Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology (Fuzhou University), Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel Farming and Processing, Fuzhou, Fujian, 350108, PR China.
Heliyon. 2024 May 10;10(11):e31060. doi: 10.1016/j.heliyon.2024.e31060. eCollection 2024 Jun 15.
Resulted from the severe side effects, the development of inexpensive, simple and sensitive method for amethopterin (ATP, an antineoplastic drug) is very important but it still remains a challenge. In this work, low cost nanohybrid composed of carbon nanobowl (CNB) and β-cyclodextrins (β-CD) (CNB-CD) was prepared with a simple autopolymerization way and applied as electrode material to develop a novel electrochemical sensor of ATP. Scanning-/transmission-electron microscopy, Fourier transform infrared spectrum, photographic image and electrochemical technologies were utilized to characterize morphologies and structure of the as-prepared CNB and CNB-CD materials. On the basic of the coordination advantages from CNB (prominent electrical property and surface area) and β-CD (superior molecule-recognition and solubility capabilities), the CNB-CD nanohybrid modified electrode exhibits superior sensing performances toward ATP, and a low detection limit of 0.002 μM coupled with larger linearity of 0.005-12.0 μM are obtained. In addition, the as-prepared sensor offers desirable repeatability, stability, selectivity and practical application property, confirming that this proposal may have important applications in the determination of ATP.
由于严重的副作用，开发一种用于氨甲蝶呤（ATP，一种抗肿瘤药物）的廉价、简单且灵敏的方法非常重要，但这仍然是一个挑战。在这项工作中，通过简单的自聚合方法制备了由碳纳米碗（CNB）和β-环糊精（β-CD）组成的低成本纳米杂化物（CNB-CD），并将其用作电极材料来开发一种新型的ATP电化学传感器。利用扫描/透射电子显微镜、傅里叶变换红外光谱、照片图像和电化学技术对所制备的CNB和CNB-CD材料的形貌和结构进行了表征。基于CNB（突出的电学性质和表面积）和β-CD（优异的分子识别和溶解能力）的配位优势，CNB-CD纳米杂化物修饰电极对ATP表现出优异的传感性能，获得了0.002 μM的低检测限以及0.005 - 12.0 μM的较大线性范围。此外，所制备的传感器具有良好的重复性、稳定性、选择性和实际应用性能，证实了该方案在ATP测定中可能具有重要应用。
