Department of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 3 East Qingchun Road, Hangzhou, Zhejiang, 310016, China.
Key Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang Province, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, 58 Shangtang Road, Hangzhou, Zhejiang, 310014, China.
Chemosphere. 2023 Aug;331:138769. doi: 10.1016/j.chemosphere.2023.138769. Epub 2023 Apr 24.
Analyzing the levels of anticancer medications in biological samples and body fluids reveals important details on the course and effects of chemotherapy. p (L-Cys)/graphitic-carbon nitride (g-CN)/GCE, a modified glassy carbon electrode, was created for the current study's electrochemical detection of methotrexate (MTX), a drug used to treat breast cancer, in pharmaceutical fluid samples. l-Cysteine was electro-polymerized on the surface of the g-CN/GCE after the g-CN was first modified to prepare the p (L-Cys)/g-CN/GCE. Analyses of morphology and structure showed that well-crystalline p (L-Cys) on g-CN/GCE was successfully electropolymerized. Studying the electrochemical characteristics of p (L-Cys)/g-CN/GCE using CV and DPV techniques revealed a synergistic impact between g-CN and l-cysteine that improved the stability and selectivity of the electrochemical oxidation of MTX while enhancing the electrochemical signal. Results showed that 7.5-780 μM was the linear range, and that 0.11841 μA/μM and 6 nM, respectively, were the sensitivity and limit of detection. The applicability of the suggested sensors was assessed using real pharmaceutical preparations, and the results showed that p (L-Cys)/g-CN/GCE had a high degree of precision. Five breast cancer patients who volunteered and provided prepared blood serum samples between the ages of 35 and 50 were used to examine the validity and accuracy of the proposed sensor in the current work for the determination of MTX. The results showed good recovery values (greater than 97.20%), appropriate accuracy (RSD less than 5.11%), and good agreement between the ELISA and DPV analysis results. These findings showed that p (L-Cys)/g-CN/GCE can be applied as a trustworthy MTX sensor for MTX level monitoring in blood samples and pharmaceutical samples.
分析生物样本和体液中的抗癌药物水平可以揭示化疗过程和效果的重要细节。本研究采用玻碳电极（GCE）修饰，制备了 L-半胱氨酸/石墨相氮化碳（g-CN）/GCE 修饰电极，用于电化学检测药物样本中的甲氨蝶呤（MTX）。首先对 g-CN 进行修饰，然后在 g-CN 表面电聚合 L-半胱氨酸，制备 p（L-Cys）/g-CN/GCE。形貌和结构分析表明，g-CN/GCE 上成功地电聚合了结晶良好的 p（L-Cys）。通过 CV 和 DPV 技术研究 p（L-Cys）/g-CN/GCE 的电化学特性表明，g-CN 和 L-半胱氨酸之间存在协同作用，提高了 MTX 电化学氧化的稳定性和选择性，同时增强了电化学信号。结果表明，线性范围为 7.5-780 μM，灵敏度和检测限分别为 0.11841 μA/μM 和 6 nM。通过真实药物制剂评估了所提出的传感器的适用性，结果表明，所提出的传感器具有很高的精度。对 5 名年龄在 35-50 岁之间的志愿者提供的制备好的血清样本进行检测，以检验本研究中提出的传感器在 MTX 测定中的有效性和准确性。结果表明，回收率较好（大于 97.20%），准确度适宜（RSD 小于 5.11%），与 ELISA 和 DPV 分析结果具有良好的一致性。这些结果表明，p（L-Cys）/g-CN/GCE 可作为一种可靠的 MTX 传感器，用于监测血液样本和药物样本中的 MTX 水平。
