College of Chemistry, Zhengzhou University, Zhengzhou 450001, China.
Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and Pharmaceutical Sciences, Marine College, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China.
J Hazard Mater. 2022 Aug 15;436:129199. doi: 10.1016/j.jhazmat.2022.129199. Epub 2022 May 21.
In this study, a novel highly sensitive colorimetric platform has been designed for malathion assay based on Fe-N/C SAzyme. The as-synthesized SAzyme can directly oxidize 3,3´,5,5´-tetramethylbenzidine (TMB) to generate blue colored oxidized TMB. L-ascorbic acid-2-phosphate (AA2P), a substrate of acid phosphatase (ACP), could be hydrolyzed to AA, thereafter inhibit the oxidization reaction of TMB, leading to a conspicuous blue color fading. With the addition of malathion hindered the ACP activity and limited the AA production, resulting in the recovery of the catalytic activity of single-atom nanozyme. Under optimized operational conditions, a novel colorimetric assay has been designed for malathion detection with LOD of 0.42 nM. Besides, quantification of malathion in environmental and food samples was achieved based on the proposed strategy. In addition, the successfully integrated paper/smartphone sensor provided sensitive, and rapid, reliable detection of malathion with a LOD of 1 nM.
在这项研究中，设计了一种基于 Fe-N/C SAzyme 的新型高灵敏度比色测定马拉硫磷的平台。所合成的 SAzyme 可以直接氧化 3,3´,5,5´-四甲基联苯胺 (TMB) 生成蓝色的氧化 TMB。抗坏血酸-2-磷酸酯 (AA2P) 是酸性磷酸酶 (ACP) 的底物，可被水解为 AA，随后抑制 TMB 的氧化反应，导致明显的蓝色褪色。马拉硫磷会抑制 ACP 的活性并限制 AA 的生成，从而恢复单原子纳米酶的催化活性。在优化的操作条件下，设计了一种新的比色测定马拉硫磷的方法，其检测限为 0.42 nM。此外，还基于该策略实现了环境和食品样品中马拉硫磷的定量分析。此外，成功集成的纸/智能手机传感器提供了敏感、快速、可靠的马拉硫磷检测，其检测限为 1 nM。
