INRAE, Pathologie Végétale, F-84140, Montfavet, France.
Department of Fundamental Microbiology, University of Lausanne, CH-1015 Lausanne, Switzerland.
J Gen Virol. 2024 Jun;105(6). doi: 10.1099/jgv.0.001990.
Relatively few phages that infect plant pathogens have been isolated and investigated. The species complex is present in various environments, including plants. It can cause major crop diseases, such as bacterial canker on apricot trees. This study presents a collection of 25 unique phage genomes that infect . These phages were isolated from apricot orchards with bacterial canker symptoms after enrichment with 21 strains of . This collection comprises mostly virulent phages, with only three being temperate. They belong to 14 genera, 11 of which are newly discovered, and 18 new species, revealing great genetic diversity within this collection. Novel DNA packaging systems have been identified bioinformatically in one of the new phage species, but experimental confirmation is required to define the precise mechanism. Additionally, many phage genomes contain numerous potential auxiliary metabolic genes with diversified putative functions. At least three phages encode genes involved in bacterial tellurite resistance, a toxic metalloid. This suggests that viruses could play a role in bacterial stress tolerance. This research emphasizes the significance of continuing the search for new phages in the agricultural ecosystem to unravel novel ecological diversity and new gene functions. This work contributes to the foundation for future fundamental and applied research on phages infecting phytopathogenic bacteria.
相对较少的感染植物病原体的噬菌体被分离和研究。该种复合体存在于各种环境中，包括植物。它可导致主要作物病害，如杏树细菌性溃疡。本研究介绍了一组 25 种独特的噬菌体基因组，可感染。这些噬菌体是从细菌性溃疡症状的杏园果园中分离出来的，经过 21 株的富集。该集合主要由毒性噬菌体组成，只有三种是温和的。它们属于 14 个属，其中 11 个是新发现的，18 个新物种，揭示了该集合内的巨大遗传多样性。在一种新的噬菌体物种中，通过生物信息学鉴定出了新的 DNA 包装系统，但需要实验验证来确定精确的机制。此外，许多噬菌体基因组包含许多具有多样化假定功能的潜在辅助代谢基因。至少有三种噬菌体编码与细菌碲酸盐抗性相关的基因，碲酸盐是一种有毒的类金属。这表明病毒可能在细菌的应激耐受中发挥作用。这项研究强调了在农业生态系统中继续寻找新噬菌体的重要性，以揭示新的生态多样性和新的基因功能。这项工作为未来感染植物病原菌的噬菌体的基础和应用研究奠定了基础。
