Department of Medicine, University of California, San Diego, School of Medicine, La Jolla, CA 92093, USA.
Department of Medicine, University of California, San Diego, School of Medicine, La Jolla, CA 92093, USA; Department of Surgery, University of California, San Diego, School of Medicine, La Jolla, CA 92093, USA.
STAR Protoc. 2024 Jun 21;5(2):103111. doi: 10.1016/j.xpro.2024.103111. Epub 2024 Jun 3.
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic "livers in a dish." We describe strategies to induce metabolic and alcohol-associated hepatic steatosis, inflammation, and fibrosis. We outline potential applications, including using human liver spheroids for experimental and translational research and drug screening to identify potential anti-fibrotic therapies.
目前，肥胖和酒精性肝病尚无有效的治疗方法，部分原因是缺乏转化型人类模型。在此，我们提出了一种生成包含所有肝细胞类型并模拟“盘中之肝”的 3D 人类肝球体的方案。我们描述了诱导代谢和酒精相关性肝脂肪变性、炎症和纤维化的策略。我们概述了潜在的应用，包括使用人类肝球体进行实验和转化研究以及药物筛选，以确定潜在的抗纤维化治疗方法。
