大肠杆菌RNA聚合酶与核糖体RNA启动子之间的相互作用。
Interaction between RNA polymerase and a ribosomal RNA promoter of E. coli.
作者信息
Hamming J, Gruber M, AB G
出版信息
Nucleic Acids Res. 1979 Oct 25;7(4):1019-33. doi: 10.1093/nar/7.4.1019.
Abstract
The interaction between RNA polymerase and the E. coli ribosomal (r) RNA promoter(s) of the rrnE operon has been studied by the filter-binding method. The extent of complex formation between RNA polymerase and rrnE promoter(s) is salt-dependent; ppGpp specifically inhibits interaction of RNA polymerase with the rrnE promoter(s). A tentative model is proposed for the molecular events in the early steps of rRNA initiation: a transition of the primarily formed, labile RNA polymerase-rRNA promoter complex to a more stable form is the determining step. This step is salt-sensitive; ppGpp acts on this "isomerization".
摘要
通过滤膜结合法研究了RNA聚合酶与rrnE操纵子的大肠杆菌核糖体（r）RNA启动子之间的相互作用。RNA聚合酶与rrnE启动子之间形成复合物的程度取决于盐浓度；ppGpp特异性抑制RNA聚合酶与rrnE启动子的相互作用。针对rRNA起始早期步骤中的分子事件提出了一个初步模型：最初形成的不稳定的RNA聚合酶-rRNA启动子复合物向更稳定形式的转变是决定性步骤。这一步骤对盐敏感；ppGpp作用于这种“异构化”。
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