Tabak H F, Flavell R A
Nucleic Acids Res. 1978 Jul;5(7):2321-32. doi: 10.1093/nar/5.7.2321.
We describe a quick and versatile method for the isolation of DNA from agarose gels. The DNA is electrophoresed into a trough containing hydroxyapatite, where it is bound. The hydroxyapatite is taken out and the DNA eluted with phosphate buffer. By putting the hydroxyapatite on a small column of Sephadex G50, elution and subsequent removal of phosphate can be performed in one step. The DNA recovered can be used equally well in enzymatic incubations as DNA not purified through agarose gel electrophoresis. Several applications of this technique are described.
我们描述了一种从琼脂糖凝胶中分离DNA的快速且通用的方法。将DNA电泳至含有羟基磷灰石的槽中，使其结合。取出羟基磷灰石，并用磷酸盐缓冲液洗脱DNA。通过将羟基磷灰石置于一小柱Sephadex G50上，洗脱及随后去除磷酸盐可一步完成。回收的DNA在酶促反应中的使用效果与未通过琼脂糖凝胶电泳纯化的DNA一样好。本文描述了该技术的几种应用。
