Appel B, Erdmann V A, Stulz J, Ackerman T
Nucleic Acids Res. 1979 Oct 25;7(4):1043-57. doi: 10.1093/nar/7.4.1043.
The extent of base pairing in Escherichia coli and Bacillus stearothermophilus 5S RNAs was determined by infrared spectroscopy. From the infrared spectra taken at 20 degrees and 52 degrees C it is concluded that E. coli and B. stearothermophlius 5S RNAs possess a large number of base pairs (Table I). Comparison of our results with those previously published using other methods leads to the conclusion that the structures of prokaryotic 5S RNAs involve a large number of tertiary interactions, in which the base pairing is not necessarily solely of the Watson-Crick type.
通过红外光谱法测定了大肠杆菌和嗜热脂肪芽孢杆菌5S RNA中的碱基配对程度。从在20℃和52℃下测得的红外光谱可以得出结论,大肠杆菌和嗜热脂肪芽孢杆菌5S RNA拥有大量碱基对(表I)。将我们的结果与之前使用其他方法发表的结果进行比较,得出的结论是原核生物5S RNA的结构涉及大量三级相互作用,其中碱基配对不一定仅是沃森-克里克类型。