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通过红外光谱法测定大肠杆菌和嗜热脂肪芽孢杆菌5S RNA中的碱基配对

Determination of base pairing in Escherichia coli and Bacillus stearothermophilus 5S RNAs by infrared spectroscopy.

作者信息

Appel B, Erdmann V A, Stulz J, Ackerman T

出版信息

Nucleic Acids Res. 1979 Oct 25;7(4):1043-57. doi: 10.1093/nar/7.4.1043.

Abstract

The extent of base pairing in Escherichia coli and Bacillus stearothermophilus 5S RNAs was determined by infrared spectroscopy. From the infrared spectra taken at 20 degrees and 52 degrees C it is concluded that E. coli and B. stearothermophlius 5S RNAs possess a large number of base pairs (Table I). Comparison of our results with those previously published using other methods leads to the conclusion that the structures of prokaryotic 5S RNAs involve a large number of tertiary interactions, in which the base pairing is not necessarily solely of the Watson-Crick type.

摘要

通过红外光谱法测定了大肠杆菌和嗜热脂肪芽孢杆菌5S RNA中的碱基配对程度。从在20℃和52℃下测得的红外光谱可以得出结论,大肠杆菌和嗜热脂肪芽孢杆菌5S RNA拥有大量碱基对(表I)。将我们的结果与之前使用其他方法发表的结果进行比较,得出的结论是原核生物5S RNA的结构涉及大量三级相互作用,其中碱基配对不一定仅是沃森-克里克类型。

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本文引用的文献

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Possible conformations of 5-S ribosomal RNA.5-S核糖体RNA的可能构象
Nature. 1967 Nov 4;216(5114):513-4. doi: 10.1038/216513a0.
5
Interbase vibrational coupling in G:C polynucleotide helices.G:C多核苷酸螺旋中的碱基间振动耦合。
Proc Natl Acad Sci U S A. 1969 Oct;64(2):451-8. doi: 10.1073/pnas.64.2.451.
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Computer generation of pairing schemes for RNA molecules.RNA分子配对方案的计算机生成
J Theor Biol. 1972 Feb;34(2):363-78. doi: 10.1016/0022-5193(72)90168-3.
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Primary structure of RNA.RNA的一级结构
Annu Rev Biochem. 1968;37:131-48. doi: 10.1146/annurev.bi.37.070168.001023.

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