Stulz J, Ackermann T, Appel B, Erdmann V A
Nucleic Acids Res. 1981 Aug 11;9(15):3851-61. doi: 10.1093/nar/9.15.3851.
Infrared Spectroscopy was used to determine the numbers of base pairs for yeast 5S RNA and 5.8S RNA. The spectra were recorded at 20 degrees C and 50 degrees C, where tertiary interactions are assumed to be of less importance. It may be concluded that the structure of both RNAs is highly ordered and that there are large contributions of tertiary interactions. The results are compared with data derived from structural models that were proposed in the literature as well as with data previously published for prokaryotic 5S RNAs.
红外光谱法用于测定酵母5S RNA和5.8S RNA的碱基对数。光谱在20摄氏度和50摄氏度下记录,此时假定三级相互作用的重要性较低。可以得出结论,两种RNA的结构都是高度有序的,并且三级相互作用有很大贡献。将结果与文献中提出的结构模型得出的数据以及先前发表的原核5S RNA的数据进行了比较。