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酵母5S和5.8S RNA碱基配对的红外光谱测定

Determination of base pairing in yeast 5S and 5.8S RNA infrared spectroscopy.

作者信息

Stulz J, Ackermann T, Appel B, Erdmann V A

出版信息

Nucleic Acids Res. 1981 Aug 11;9(15):3851-61. doi: 10.1093/nar/9.15.3851.

DOI:10.1093/nar/9.15.3851
PMID:7024920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327396/
Abstract

Infrared Spectroscopy was used to determine the numbers of base pairs for yeast 5S RNA and 5.8S RNA. The spectra were recorded at 20 degrees C and 50 degrees C, where tertiary interactions are assumed to be of less importance. It may be concluded that the structure of both RNAs is highly ordered and that there are large contributions of tertiary interactions. The results are compared with data derived from structural models that were proposed in the literature as well as with data previously published for prokaryotic 5S RNAs.

摘要

红外光谱法用于测定酵母5S RNA和5.8S RNA的碱基对数。光谱在20摄氏度和50摄氏度下记录，此时假定三级相互作用的重要性较低。可以得出结论，两种RNA的结构都是高度有序的，并且三级相互作用有很大贡献。将结果与文献中提出的结构模型得出的数据以及先前发表的原核5S RNA的数据进行了比较。

相似文献

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Determination of base pairing in yeast 5S and 5.8S RNA infrared spectroscopy.酵母5S和5.8S RNA碱基配对的红外光谱测定
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引用本文的文献

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Melting of local ordered structures in yeast 5S ribosomal RNA in aqueous salts.酵母5S核糖体RNA在盐水溶液中局部有序结构的熔化
Nucleic Acids Res. 1983 May 25;11(10):3363-73. doi: 10.1093/nar/11.10.3363.
2
The 5S RNA genes of Schizosaccharomyces pombe.粟酒裂殖酵母的5S RNA基因。
Nucleic Acids Res. 1982 Jan 22;10(2):487-500. doi: 10.1093/nar/10.2.487.
3
RNA structure analysis using T2 ribonuclease: detection of pH and metal ion induced conformational changes in yeast tRNAPhe.使用T2核糖核酸酶进行RNA结构分析：检测pH值和金属离子诱导的酵母苯丙氨酸tRNA构象变化。
Nucleic Acids Res. 1984 Sep 11;12(17):6763-78. doi: 10.1093/nar/12.17.6763.
4
Three-dimensional structural model of eubacterial 5S RNA that has functional implications.具有功能意义的真细菌5S RNA三维结构模型。
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4599-603. doi: 10.1073/pnas.79.15.4599.

本文引用的文献

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The purine and pyrimidine composition of deoxypentose nucleic acids.脱氧戊糖核酸的嘌呤和嘧啶组成。
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Enzymic synthesis of polynucleotides. I. Polynucleotide phosphorylase of azotobacter vinelandii.多核苷酸的酶促合成。I. 棕色固氮菌的多核苷酸磷酸化酶。
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Melting of Saccharomyces cerevisiae 5S ribonucleic acid: ultraviolet absorption, circular dichroism, and 360-MHz proton nuclear magnetic resonance spectroscopy.酿酒酵母5S核糖核酸的熔解：紫外吸收、圆二色性和360兆赫兹质子核磁共振光谱法
Biochemistry. 1980 Nov 11;19(23):5456-62. doi: 10.1021/bi00564a047.
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Physicochemical studies on the 5S ribonucleic acid-protein complex from a eucaryote, Saccharomyces cerevisiae.对真核生物酿酒酵母的5S核糖核酸-蛋白质复合物的物理化学研究。
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Determination of the base pairing content of ribonucleic acids by infrared spectroscopy.用红外光谱法测定核糖核酸的碱基配对含量
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Polynucleotides. VI. Interaction between polyguanylic acid and polycytidylic acid.多核苷酸。六、聚鸟苷酸与聚胞苷酸之间的相互作用。
Proc Natl Acad Sci U S A. 1965 Jun;53(6):1425-30. doi: 10.1073/pnas.53.6.1425.
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Amount of adenine and uracil base pairs in E. coli 23S, 16S and 5S ribosomal RNA.大肠杆菌23S、16S和5S核糖体RNA中腺嘌呤和尿嘧啶碱基对的数量。
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Oligonucleotide binding to the native and denatured conformers of yeast transfer RNA-3 Lea.寡核苷酸与酵母转移RNA - 3 Lea的天然和变性构象的结合
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Nucleotide sequence of 5 S RNA from Torulopsis utilis.产朊假丝酵母5S RNA的核苷酸序列
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