Jilin Agricultural University (JLAU)-Borui Dairy Science and Technology R&D Center, Key Laboratory of Animal Nutrition and Feed Science of Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Key Laboratory of Animal Production Product Quality and Security Ministry of Education, College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Front Immunol. 2024 May 21;15:1398310. doi: 10.3389/fimmu.2024.1398310. eCollection 2024.
Hydroxytryptophan (5-HTP) can regulate the synthesis of 5-Hydroxytryptamine (5-HT) and melatonin (MT). In a previous metabolome analysis, we found that 5-HTP is an effective ingredient in yeast culture for regulating rumen fermentation. However, research on the effect of this microbial product (5-HTP) as a functional feed additive in sheep production is still not well explained. Therefore, this study examined the effects of 5-HTP on sheep rumen function and growth performance using and models.
A two-factor in vitro experiment involving different 5-HTP doses and fermentation times was conducted. Then, in the experiment, 10 sheep were divided into a control group which was fed a basal diet, and a 5-HTP group supplemented with 8 mg/kg 5-HTP for 60 days.
The results showed that 5-HTP supplementation had a significant effect on DMD, pH, NH-N, acetic acid, propionic acid, and TVFA concentrations. 5-HTP altered rumen bacteria composition and diversity indices including Chao1, Shannon, and Simpson. Moreover, the study on sheep confirmed that supplementing with 8 mg/kg of 5-HTP improved rumen fermentation efficiency and microbial composition. This led to enhanced sheep growth performance and increased involvement in the tryptophan metabolic pathway, suggesting potential benefits.
Dietary 5-HTP (8 mg/kg DM) improves sheep growth performance by enhancing ruminal functions, antioxidant capacity, and tryptophan metabolism. This study can provide a foundation for the development of 5-HTP as a functional feed additive in ruminants' production.
羟基色氨酸（5-HTP）可以调节 5-羟色氨酸（5-HT）和褪黑素（MT）的合成。在之前的代谢组学分析中，我们发现 5-HTP 是一种调节瘤胃发酵的酵母培养物的有效成分。然而，作为绵羊生产中功能性饲料添加剂的这种微生物产品（5-HTP）的研究仍未得到很好的解释。因此，本研究使用 和 模型研究了 5-HTP 对绵羊瘤胃功能和生长性能的影响。
进行了一项涉及不同 5-HTP 剂量和发酵时间的双因素体外试验。然后，在 试验中，将 10 只绵羊分为对照组，饲喂基础日粮，5-HTP 组在基础日粮中添加 8mg/kg 5-HTP，试验期 60 天。
结果表明，5-HTP 补充剂对 DMD、pH、NH3-N、乙酸、丙酸和 TVFA 浓度有显著影响。5-HTP 改变了瘤胃细菌组成和多样性指数，包括 Chao1、Shannon 和 Simpson。此外，绵羊的 研究证实，补充 8mg/kg 的 5-HTP 可提高瘤胃发酵效率和微生物组成。这导致绵羊生长性能提高，并增加了色氨酸代谢途径的参与，表明具有潜在的益处。
日粮 5-HTP（8mg/kg DM）通过增强瘤胃功能、抗氧化能力和色氨酸代谢来提高绵羊的生长性能。本研究可为 5-HTP 作为反刍动物生产中功能性饲料添加剂的开发提供基础。
