Department of Critical Care Medicine, The First Affiliated Hospital of Harbin Medical University, No. 23 Youzheng Street, Harbin, 150001, Heilongjiang Province, China.
Department of Critical Care Medicine, Harbin Medical University Cancer Hospital, Harbin, 150000, China.
Int Microbiol. 2023 Nov;26(4):929-938. doi: 10.1007/s10123-023-00349-x. Epub 2023 Mar 27.
Sepsis causes high mortality in intensive care units. Although there have been many studies on the gut microbiota in patients with sepsis, the impact of sepsis on the gut microbiota has not been directly determined because the treatment of sepsis also affects the gut microbiota. Therefore, we designed this animal experiment to explore gut microbiota alterations during sepsis. Mice were divided into two groups, mice that survived less than 3 days and mice that survived more than 3 days. Fecal samples collected on the day of cecal ligation and puncture (CLP), as well as on the 3rd and 7th days after CLP, were subjected to microbial community analysis and nontargeted metabolomics analysis. The results showed significantly lower bacterial diversity in fecal samples after CLP. At the genus level, the fecal samples obtained on the 3rd and 7th days after CLP exhibited significantly increased relative abundances of Bacteroides, Helicobacter, etc., and significantly decreased relative abundances of Alloprevotella, Prevotella, etc. Innate metabolite levels were significantly different in mice that survived less than 3 days and mice that survived more than 3 days. In conclusion, CLP-induced sepsis in mice changes the structure of the gut microbiome, and innate metabolites affect the prognosis of septic mice.
脓毒症会导致重症监护病房的死亡率升高。虽然已经有许多关于脓毒症患者肠道微生物群的研究，但由于脓毒症的治疗也会影响肠道微生物群，因此尚未直接确定脓毒症对肠道微生物群的影响。因此，我们设计了这项动物实验，以探讨脓毒症期间肠道微生物群的变化。将小鼠分为两组，存活时间少于 3 天的小鼠和存活时间超过 3 天的小鼠。在盲肠结扎和穿刺（CLP）当天以及 CLP 后第 3 天和第 7 天收集粪便样本，进行微生物群落分析和非靶向代谢组学分析。结果表明，CLP 后粪便样本中的细菌多样性明显降低。在属水平上，CLP 后第 3 天和第 7 天获得的粪便样本中，Bacteroides、Helicobacter 等的相对丰度显著增加，而 Alloprevotella、Prevotella 等的相对丰度显著降低。存活时间少于 3 天和存活时间超过 3 天的小鼠之间的先天代谢物水平存在显著差异。总之，CLP 诱导的小鼠脓毒症改变了肠道微生物组的结构，先天代谢物影响脓毒症小鼠的预后。
