School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, Gansu, China.
Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, Gansu, China.
Braz J Microbiol. 2024 Sep;55(3):2411-2422. doi: 10.1007/s42770-024-01392-0. Epub 2024 Jun 5.
Microbial remediation plays a pivotal role in the elimination of petroleum pollutants, making it imperative to investigate the capabilities of microorganisms in degrading petroleum. The present study describes the isolation of a promising strain, Acinetobacter sp. HX09, from petroleum-contaminated water. GC-MS analysis revealed a remarkable removal efficiency for short and medium-chain alkanes, with a rate of approximately 64% after a 7-days incubation at 30 °C. Transcriptome analysis of HX09 exhibited a predominant upregulation in gene expression levels by the induce of crude oil. Notably, genes such as alkane 1-monooxygenase, dehydrogenases and fatty acid metabolic enzymes exhibited fold changes range from 3.16 to 1.3. Based on the alkB gene sequences in HX09, the Phyre2 algorithm generated a three-dimensional structure that exhibited similarity to segments of acyl coenzyme desaturases and acyl lipid desaturases. Furthermore, three biodegradation-related gene clusters were predicted in HX09 based on the reference genome sequence. These findings contribute to our understanding of the hydrocarbon-degrading mechanisms employed by Acinetobacter species and facilitate the development of effective remediation strategies for crude oil- polluted environments.
微生物修复在石油污染物的消除中起着关键作用，因此必须研究微生物降解石油的能力。本研究从石油污染水中分离出一种有前途的菌株，不动杆菌属 HX09。GC-MS 分析表明，该菌株在 30°C 孵育 7 天后，对短链和中链烷烃的去除效率非常高，达到约 64%。HX09 的转录组分析表明，在原油的诱导下，其基因表达水平显著上调。值得注意的是，烷烃 1-单加氧酶、脱氢酶和脂肪酸代谢酶等基因的表达水平变化倍数在 3.16 到 1.3 之间。基于 HX09 中的 alkB 基因序列，Phyre2 算法生成了一个三维结构，与酰基辅酶脱氢酶和酰基脂质脱氢酶的片段具有相似性。此外，根据参考基因组序列，在 HX09 中预测了三个与生物降解相关的基因簇。这些发现有助于我们了解不动杆菌属利用烃类的降解机制，并为开发有效的原油污染环境修复策略提供了依据。
