Hong Yue-Hui, Deng Mao-Cheng, Xu Xiao-Ming, Wu Chou-Fei, Xiao Xi, Zhu Qing, Sun Xian-Xian, Zhou Qian-Zhi, Peng Juan, Yuan Jian-Ping, Wang Jiang-Hai
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, PR China; South China Sea Bioresource Exploitation and Utilization Collaborative Innovation Center, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, PR China.
Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, PR China; South China Sea Bioresource Exploitation and Utilization Collaborative Innovation Center, School of Marine Sciences, Sun Yat-Sen University, Guangzhou 510006, PR China; Department of Food and Bioengineering, Guangdong Industry Technical College, Guangzhou 510300, PR China.
Gene. 2016 Jun 15;584(2):185-94. doi: 10.1016/j.gene.2016.02.032. Epub 2016 Feb 23.
Microbial remediation has become one of the most important strategies for eliminating petroleum pollutants. Revealing the transcript maps of microorganisms with the hydrocarbon-degrading ability contributes to enhance the degradation of hydrocarbons and further improve the effectiveness of bioremediation. In this study, we characterized the transcriptome of hydrocarbon-degrading Achromobacter sp. HZ01 after petroleum treatment for 16h. A total of 38,706,280 and 38,954,413 clean reads were obtained by RNA-seq for the petroleum-treated group and control, respectively. By an effective de novo assembly, 3597 unigenes were obtained, including 3485 annotated transcripts. Petroleum treatment had significantly influenced the transcriptional profile of strain HZ01, involving 742 differentially expressed genes. A part of genes were activated to exert specific physiological functions, whereas more genes were down-regulated including specific genes related to cell motility, genes associated with glycometabolism, and genes coding for ribosomal proteins. Identification of genes related to petroleum degradation revealed that the fatty acid metabolic pathway and a part of monooxygenases and dehydrogenases were activated, whereas the TCA cycle was inactive. Additionally, terminal oxidation might be a major aerobic pathway for the degradation of n-alkanes in strain HZ01. The newly obtained data contribute to better understand the gene expression profiles of hydrocarbon-degrading microorganisms after petroleum treatment, to further investigate the genetic characteristics of strain HZ01 and other related species and to develop cost-effective and eco-friendly strategies for remediation of crude oil-polluted environments.
微生物修复已成为消除石油污染物的最重要策略之一。揭示具有烃降解能力的微生物的转录图谱有助于增强烃的降解，并进一步提高生物修复的效果。在本研究中，我们对石油处理16小时后的烃降解无色杆菌属菌株HZ01的转录组进行了表征。通过RNA测序，石油处理组和对照组分别获得了38,706,280和38,954,413条清洁 reads。通过有效的从头组装，获得了3597个单基因，包括3485个注释转录本。石油处理显著影响了菌株HZ01的转录谱，涉及742个差异表达基因。一部分基因被激活以发挥特定的生理功能，而更多的基因被下调，包括与细胞运动相关的特定基因、与糖代谢相关的基因以及编码核糖体蛋白的基因。与石油降解相关基因的鉴定表明，脂肪酸代谢途径以及一部分单加氧酶和脱氢酶被激活，而三羧酸循环不活跃。此外，末端氧化可能是菌株HZ01中正构烷烃降解的主要需氧途径。新获得的数据有助于更好地了解石油处理后烃降解微生物的基因表达谱，进一步研究菌株HZ01和其他相关物种的遗传特征，并开发经济高效且生态友好的原油污染环境修复策略。
