Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science & Technology, Wuhan, 430074, China; School of Chemistry, Chemical Engineering and Biotechnology, Nanyang Technological University, Singapore, 637457, Singapore.
Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science & Technology, Wuhan, 430074, China.
Biosens Bioelectron. 2024 Sep 15;260:116463. doi: 10.1016/j.bios.2024.116463. Epub 2024 Jun 2.
Studies on the interaction between hydrogen sulfide (HS) and hydrogen peroxide (HO) in redox signaling motivate the development of a sensitive sensing platform for their discriminatory and dynamic detection. Herein, we present a fully integrated microfluidic on-chip electrochemical sensor for the online and simultaneous monitoring of HS and HO secreted by different biological samples. The sensor utilizes a cicada-wing-like RuCu bimetal-organic framework with uniform nanorods architecture that grows on a flexible carbon fiber microelectrode. Owing to the optimized electronic structural merits and satisfactory electrocatalytic properties, the resultant microelectrode shows remarkable electrochemical sensing performance for sensitive and selective detection of HS and HO at the same time. The result exhibits low detection limits of 0.5 μM for HS and 0.1 μM for HO, with high sensitivities of 61.93 μA cm mM for HS, and 75.96 μA cm mM for HO. The integration of this biocompatible microelectrode into a custom wireless microfluidic chip enables the construction of a miniature intelligent system for in situ monitoring of HS and HO released from different living cells to differentiate between cancerous and normal cells. When applied for real-time tracking of HS and HO secreted by colorectal cancer tissues, it allows the evaluation of their chemotherapeutic efficacy. These findings hold paramount implications for disease diagnosis and therapy.
研究硫化氢（HS）和过氧化氢（HO）在氧化还原信号中的相互作用，促使人们开发出一种用于其区分和动态检测的灵敏传感平台。在此，我们提出了一种完全集成的微流控片上电化学传感器，用于在线和同时监测来自不同生物样品分泌的 HS 和 HO。该传感器利用了一种具有均匀纳米棒结构的蝉翼状 RuCu 双金属有机骨架，生长在柔性碳纤维微电极上。由于优化的电子结构优点和令人满意的电催化性能，所得微电极显示出出色的电化学传感性能，可同时对 HS 和 HO 进行灵敏和选择性检测。结果表明，HS 的检测限低至 0.5 μM，HO 的检测限低至 0.1 μM，HS 的灵敏度为 61.93 μA·cm·mM，HO 的灵敏度为 75.96 μA·cm·mM。将这种生物相容性微电极集成到定制的无线微流控芯片中，可构建一个微型智能系统，用于原位监测来自不同活细胞的 HS 和 HO 释放，以区分癌细胞和正常细胞。当应用于实时跟踪结直肠组织分泌的 HS 和 HO 时，可评估其化疗效果。这些发现对疾病诊断和治疗具有重要意义。
