Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science & Technology, Wuhan, 430074, China; Henan Key Laboratory of Cancer Epigenetics; Cancer Institute, The First Affiliated Hospital, and College of Clinical Medicine, Henan University of Science and Technology, Luoyang, 471003, China.
Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science & Technology, Wuhan, 430074, China.
Biosens Bioelectron. 2022 Jun 1;205:114095. doi: 10.1016/j.bios.2022.114095. Epub 2022 Feb 19.
The development of high-efficient biosensing systems for rapid and sensitive detection of disease-related biomarkers in human samples is of great significance for disease diagnosis and treatment in clinical practice. In this work, we develop an integrated electrochemical microfluidic sensing platform based on freestanding graphene fiber (GF) microelectrode for bioassay. In order to improve the electrocatalytic activity of GF microelectrode, it has been modified by unique 3D well-ordered hierarchically porous nickel-cobalt phosphide (NiCoP) nanosheet arrays (NSAs). Benefiting from the excellent electrochemical properties and structural merits, the resultant NiCoP-NSAs modified GF microelectrode shows excellent sensing performances towards neurotransmitter dopamine (DA), with a high sensitivity of 5.56 μA cm μM, a low detection limit of 14 nM, as well as good selectivity, reproducibility and stability. Furthermore, in virtue of the miniaturized size and good mechanical properties, the nanohybrid GF microelectrode can be embedded into a home-made microfluidic chip to construct an integrated electrochemical microfluidic sensing device, which has been used for sensitive analysis of DA in minimal volume of human serum and urine samples, and in situ tracking DA released from neuroblastoma cells SHSY-5Y under the stimulation for physio-pathological and pharmacological study of nervous system-related diseases.
用于快速灵敏地检测人体样本中与疾病相关的生物标志物的高效生物传感系统的开发对于临床实践中的疾病诊断和治疗具有重要意义。在这项工作中，我们开发了一种基于独立式石墨烯纤维（GF）微电极的集成电化学生物传感平台用于生物分析。为了提高 GF 微电极的电催化活性，对其进行了独特的 3D 有序分级多孔镍钴磷化物（NiCoP）纳米片阵列（NSAs）的修饰。得益于优异的电化学性能和结构优势，所得的 NiCoP-NSAs 修饰的 GF 微电极对神经递质多巴胺（DA）表现出优异的传感性能，具有 5.56 μA cm μM 的高灵敏度、14 nM 的低检测限以及良好的选择性、重现性和稳定性。此外，由于尺寸小巧和良好的机械性能，纳米杂化 GF 微电极可以嵌入到自制的微流控芯片中，构建集成电化学生物微流控传感装置，该装置已用于最小体积的人血清和尿液样本中 DA 的灵敏分析，以及神经母细胞瘤细胞 SHSY-5Y 释放的 DA 的原位追踪，用于神经系统相关疾病的生理病理和药理学研究。
