饥饿、糖尿病及胰岛素对大鼠肝脏胞质溶胶中酪蛋白激酶2的影响。
Effect of starvation, diabetes and insulin on the casein kinase 2 from rat liver cytosol.
作者信息
Martos C, Plana M, Guasch M D, Itarte E
出版信息
Biochem J. 1985 Jan 15;225(2):321-6. doi: 10.1042/bj2250321.
Abstract
Starvation, diabetes and insulin did not alter the concentration of casein kinases in rat liver cytosol. However, the Km for casein of casein kinase 2 from diabetic rats was about 2-fold lower than that from control animals. Administration of insulin to control rats did not alter this parameter, but increased the Km for casein of casein kinase 2 in diabetic rats. Starvation did not affect the kinetic constants of casein kinases. The effect of diabetes on casein kinase 2 persisted after partial purification of the enzyme by glycerol-density-gradient centrifugation and affected also its activity on other protein substrates such as phosvitin, high-mobility-group protein 14 and glycogen synthase. The results indicate that rat liver cytosol casein kinase 2 is under physiological control.
摘要
饥饿、糖尿病和胰岛素均未改变大鼠肝脏胞质溶胶中酪蛋白激酶的浓度。然而，糖尿病大鼠的酪蛋白激酶2对酪蛋白的米氏常数（Km）比对照动物的约低2倍。给对照大鼠注射胰岛素不会改变该参数，但会增加糖尿病大鼠中酪蛋白激酶2对酪蛋白的Km。饥饿不影响酪蛋白激酶的动力学常数。通过甘油密度梯度离心对该酶进行部分纯化后，糖尿病对酪蛋白激酶2的影响依然存在，并且该影响也作用于其对其他蛋白质底物（如卵黄高磷蛋白、高迁移率族蛋白14和糖原合酶）的活性。结果表明大鼠肝脏胞质溶胶中的酪蛋白激酶2受生理调控。
相似文献
1
Effect of starvation, diabetes and insulin on the casein kinase 2 from rat liver cytosol.饥饿、糖尿病及胰岛素对大鼠肝脏胞质溶胶中酪蛋白激酶2的影响。
Biochem J. 1985 Jan 15;225(2):321-6. doi: 10.1042/bj2250321.
2
Effect of bivalent cations on rat liver cytosol casein (glycogen synthase) kinases 1 and 2. Influence of the protein substrate.二价阳离子对大鼠肝脏胞质溶胶酪蛋白（糖原合酶）激酶1和激酶2的影响。蛋白质底物的作用。
Biochem J. 1985 Aug 15;230(1):69-74. doi: 10.1042/bj2300069.
3
Purification and characterization of two cyclic AMP-independent casein/glycogen synthase kinases from rat liver cytosol.大鼠肝细胞溶质中两种非环磷酸腺苷依赖性酪蛋白/糖原合酶激酶的纯化与鉴定
Biochim Biophys Acta. 1981 Apr 14;658(2):334-47. doi: 10.1016/0005-2744(81)90304-1.
4
Purification and characterization of the cytoplasmic casein kinase I from rat liver.大鼠肝脏细胞质酪蛋白激酶I的纯化与特性分析
Biochim Biophys Acta. 1982 Jan 18;700(2):221-8. doi: 10.1016/0167-4838(82)90102-9.
5
Casein and histone kinases of a rat ascites hepatoma as compared with those of rat liver.大鼠腹水肝癌的酪蛋白激酶和组蛋白激酶与大鼠肝脏的酪蛋白激酶和组蛋白激酶的比较。
Jpn J Cancer Res. 1985 Dec;76(12):1154-61.
6
Acute effects of insulin and glucagon on hepatic casein kinase 2 in adult fed rats: correlation of the effects on casein kinase 2 with the changes in glycogen synthase activity.胰岛素和胰高血糖素对成年喂食大鼠肝脏酪蛋白激酶2的急性影响：酪蛋白激酶2的影响与糖原合酶活性变化的相关性。
Arch Biochem Biophys. 1989 Dec;275(2):478-85. doi: 10.1016/0003-9861(89)90394-9.
7
Casein kinases and their protein substrates in rat liver cytosol: evidence for their participation in multimolecular systems.大鼠肝细胞溶胶中的酪蛋白激酶及其蛋白质底物：它们参与多分子系统的证据。
Biochim Biophys Acta. 1985 Aug 30;846(2):248-56. doi: 10.1016/0167-4889(85)90072-2.
8
Thyroid hormone increases type I adenosine 3', 5'-monophosphate-dependent protein kinase and casein kinase activities in rat liver cytosol: analysis of protein kinases by polyacrylamide disc gel electrophoresis.甲状腺激素增加大鼠肝细胞溶胶中I型腺苷3'，5'-单磷酸依赖性蛋白激酶和酪蛋白激酶活性：通过聚丙烯酰胺圆盘凝胶电泳分析蛋白激酶。
Endocrinology. 1983 Apr;112(4):1427-33. doi: 10.1210/endo-112-4-1427.
9
Developmental changes in rat hepatic casein kinases 1 and 2.大鼠肝脏酪蛋白激酶1和2的发育变化
Eur J Biochem. 1987 Dec 30;170(1-2):493-8. doi: 10.1111/j.1432-1033.1987.tb13726.x.
10
Phosphorylation of fibrinogen by casein kinase 2.酪蛋白激酶2对纤维蛋白原的磷酸化作用。
Biochem J. 1986 Mar 15;234(3):523-6. doi: 10.1042/bj2340523.
引用本文的文献
1
Protein kinase CK2: a potential therapeutic target for diverse human diseases.蛋白激酶 CK2：一种针对多种人类疾病的潜在治疗靶点。
Signal Transduct Target Ther. 2021 May 17;6(1):183. doi: 10.1038/s41392-021-00567-7.
2
Insulin resistance induces posttranslational hepatic sortilin 1 degradation in mice.胰岛素抵抗诱导小鼠肝脏中sortilin 1的翻译后降解。
J Biol Chem. 2015 May 1;290(18):11526-36. doi: 10.1074/jbc.M115.641225. Epub 2015 Mar 23.
3
Effect of bivalent cations on rat liver cytosol casein (glycogen synthase) kinases 1 and 2. Influence of the protein substrate.二价阳离子对大鼠肝脏胞质溶胶酪蛋白（糖原合酶）激酶1和激酶2的影响。蛋白质底物的作用。
Biochem J. 1985 Aug 15;230(1):69-74. doi: 10.1042/bj2300069.
4
Phosphorylation of fibrinogen by casein kinase 2.酪蛋白激酶2对纤维蛋白原的磷酸化作用。
Biochem J. 1986 Mar 15;234(3):523-6. doi: 10.1042/bj2340523.
5
Effects of vanadate on protein kinases in rat hepatocytes.钒酸盐对大鼠肝细胞中蛋白激酶的影响。
Biochem J. 1989 Sep 1;262(2):563-7. doi: 10.1042/bj2620563.
6
Heterogeneity of rat liver cytosol casein kinase 2. Association between the alpha/alpha' -subunits of casein kinase 2 and the phosphorylatable protein pp49.大鼠肝脏胞质溶胶酪蛋白激酶2的异质性。酪蛋白激酶2的α/α'亚基与可磷酸化蛋白pp49之间的关联。
Biochem J. 1991 Aug 1;277 ( Pt 3)(Pt 3):811-8. doi: 10.1042/bj2770811.
本文引用的文献
1
An o-toluidine method for body-fluid glucose determination.一种用于测定体液葡萄糖的邻甲苯胺法。
Clin Chem. 1962 May-Jun;8:215-35.
2
A heparin-sensitive nuclear protein kinase. Purification, properties, and increased activity in rat hepatoma relative to liver.一种对肝素敏感的核蛋白激酶。纯化、性质以及与肝脏相比大鼠肝癌中活性的增加。
J Biol Chem. 1981 Jul 25;256(14):7468-77.
3
Modulators of rat liver cytosol casein kinases 1 and 2.大鼠肝脏胞质溶胶酪蛋白激酶1和2的调节剂
Biochem Biophys Res Commun. 1982 Dec 31;109(4):1284-90. doi: 10.1016/0006-291x(82)91916-7.
4
Rat liver cytosol contains an inhibitor of the casein kinases 1 and 2 from the same source.大鼠肝脏胞质溶胶含有来自同一来源的酪蛋白激酶1和2的抑制剂。
FEBS Lett. 1981 Feb 23;124(2):261-4. doi: 10.1016/0014-5793(81)80151-2.
5
Multisite phosphorylation of glycogen synthase from rabbit skeletal muscle. Phosphorylation of site 5 by glycogen synthase kinase-5 (casein kinase-II) is a prerequisite for phosphorylation of sites 3 by glycogen synthase kinase-3.兔骨骼肌糖原合酶的多位点磷酸化。糖原合酶激酶-5（酪蛋白激酶-II）对位点5的磷酸化是糖原合酶激酶-3对位点3磷酸化的前提条件。
FEBS Lett. 1982 Dec 13;150(1):191-6. doi: 10.1016/0014-5793(82)81332-x.
6
Casein kinases--multipotential protein kinases.酪蛋白激酶——多潜能蛋白激酶。
Curr Top Cell Regul. 1982;21:101-27.
7
Characterization of a rabbit skeletal muscle protein kinase (PC0.7) able to phosphorylate glycogen synthase and phosvitin.一种能够磷酸化糖原合酶和磷蛋白的兔骨骼肌蛋白激酶（PC0.7）的特性分析。
J Biol Chem. 1981 Sep 10;256(17):8955-62.
8
9
Cyclic adenosine 3':5'-monophosphate-dependent and -independent protein kinases in human leukemic cells.人白血病细胞中依赖和不依赖环磷酸腺苷（cAMP）的蛋白激酶
Cancer Res. 1983 Mar;43(3):1172-5.
10
Identification of a calmodulin-dependent glycogen synthase kinase in rabbit skeletal muscle, distinct from phosphorylase kinase.在兔骨骼肌中鉴定出一种钙调蛋白依赖性糖原合酶激酶，它与磷酸化酶激酶不同。
FEBS Lett. 1982 Nov 1;148(1):5-11. doi: 10.1016/0014-5793(82)81231-3.
Zotero 插件