Khaitovich A G, L'vovskii E A
Vopr Virusol. 1975 Mar-Apr(2):183-6.
Interferon-like virus inhibitors appeared in the blood serum of animals and in the culture fluid 15 min.--1 hour and 4-6 hours after administration of AET (S, beta-amino-ethylisotiuronium) and cystaphos (monosodium salt of beta-aminoethylthiophosphorus acid). These inhibitors showed species-specificity, were inactivated by trypsin and by heating at 37 degrees C for 2 hours and at 56 degrees C for 1 hour, did not sediment at 100,000 g. It is assumed that these are two different inhibitors of which the one forming the early peak pre-exists in the cell. It is probably associated with cell membranes and is released as a result of chemical effect of aminothyols. The late peak of inhibitor is synthesized de novo as inducated by its sensitivity to the effect of puromycin and cycloheximide.
在给予AET（S，β-氨基乙基异硫脲）和胱胺磷（β-氨基乙基硫代磷酸单钠盐）后15分钟至1小时以及4至6小时，动物血清和培养液中出现了类干扰素病毒抑制剂。这些抑制剂具有种属特异性，可被胰蛋白酶以及在37℃加热2小时和在56℃加热1小时灭活，在100,000g离心时不沉淀。据推测，这是两种不同的抑制剂，其中形成早期峰值的一种在细胞中预先存在。它可能与细胞膜相关，并由于氨基硫醇的化学作用而释放。抑制剂的晚期峰值是重新合成的，这由其对嘌呤霉素和环己酰亚胺作用的敏感性所表明。
