• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

ZNPs 通过 mTORC1-TFEB-BLOC1S3 轴促进桥粒钙黏附蛋白内吞作用来降低表皮机械应变阻力。

ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.

机构信息

Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, 510515, China.

Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

出版信息

J Nanobiotechnology. 2024 Jun 5;22(1):312. doi: 10.1186/s12951-024-02519-z.

DOI:10.1186/s12951-024-02519-z
PMID:38840221
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11151536/
Abstract

Zinc oxide nanoparticles (ZNPs) are widely used in sunscreens and nanomedicines, and it was recently confirmed that ZNPs can penetrate stratum corneum into deep epidermis. Therefore, it is necessary to determine the impact of ZNPs on epidermis. In this study, ZNPs were applied to mouse skin at a relatively low concentration for one week. As a result, desmosomes in epidermal tissues were depolymerized, epidermal mechanical strain resistance was reduced, and the levels of desmosomal cadherins were decreased in cell membrane lysates and increased in cytoplasmic lysates. This finding suggested that ZNPs promote desmosomal cadherin endocytosis, which causes desmosome depolymerization. In further studies, ZNPs were proved to decrease mammalian target of rapamycin complex 1 (mTORC1) activity, activate transcription factor EB (TFEB), upregulate biogenesis of lysosome-related organelle complex 1 subunit 3 (BLOC1S3) and consequently promote desmosomal cadherin endocytosis. In addition, the key role of mTORC1 in ZNP-induced decrease in mechanical strain resistance was determined both in vitro and in vivo. It can be concluded that ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via the mTORC1-TFEB-BLOC1S3 axis. This study helps elucidate the biological effects of ZNPs and suggests that ZNPs increase the risk of epidermal fragmentation.

摘要

氧化锌纳米粒子（ZNPs）广泛应用于防晒霜和纳米药物中，最近有研究证实 ZNPs 可以穿透角质层进入深部表皮。因此，有必要确定 ZNPs 对表皮的影响。在这项研究中，将 ZNPs 以相对较低的浓度应用于小鼠皮肤一周。结果表明，表皮组织中的桥粒解聚，表皮机械应变阻力降低，细胞膜裂解液中桥粒钙黏蛋白水平降低，细胞质裂解液中桥粒钙黏蛋白水平升高。这表明 ZNPs 促进桥粒钙黏蛋白内吞作用，导致桥粒解聚。进一步的研究表明，ZNPs 降低了哺乳动物雷帕霉素靶蛋白复合物 1（mTORC1）的活性，激活转录因子 EB（TFEB），上调溶酶体相关细胞器复合物 1 亚基 3（BLOC1S3）的生物发生，从而促进桥粒钙黏蛋白内吞作用。此外，在体外和体内均证实了 mTORC1 在 ZNP 诱导的机械应变阻力降低中的关键作用。可以得出结论，ZNPs 通过 mTORC1-TFEB-BLOC1S3 轴促进桥粒钙黏蛋白内吞作用，从而降低表皮的机械应变阻力。本研究有助于阐明 ZNPs 的生物学效应，并表明 ZNPs 增加了表皮碎片的风险。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/d061f2e45536/12951_2024_2519_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/7f4d2dd809cb/12951_2024_2519_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/db21d85aaa6d/12951_2024_2519_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/9291742cd1d7/12951_2024_2519_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/ef4b399e0a33/12951_2024_2519_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/eaad0e8472ab/12951_2024_2519_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/03b245e9e7f2/12951_2024_2519_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/d061f2e45536/12951_2024_2519_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/7f4d2dd809cb/12951_2024_2519_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/db21d85aaa6d/12951_2024_2519_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/9291742cd1d7/12951_2024_2519_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/ef4b399e0a33/12951_2024_2519_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/eaad0e8472ab/12951_2024_2519_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/03b245e9e7f2/12951_2024_2519_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bc93/11151536/d061f2e45536/12951_2024_2519_Fig7_HTML.jpg

相似文献

1
ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis.ZNPs 通过 mTORC1-TFEB-BLOC1S3 轴促进桥粒钙黏附蛋白内吞作用来降低表皮机械应变阻力。
J Nanobiotechnology. 2024 Jun 5;22(1):312. doi: 10.1186/s12951-024-02519-z.
2
TFEB-driven endocytosis coordinates MTORC1 signaling and autophagy.TFEB 驱动的内吞作用协调 MTORC1 信号和自噬。
Autophagy. 2019 Jan;15(1):151-164. doi: 10.1080/15548627.2018.1511504. Epub 2018 Sep 10.
3
Trehalose causes low-grade lysosomal stress to activate TFEB and the autophagy-lysosome biogenesis response.海藻糖会引起低水平的溶酶体应激，从而激活 TFEB 和自噬溶酶体生物发生反应。
Autophagy. 2021 Nov;17(11):3740-3752. doi: 10.1080/15548627.2021.1896906. Epub 2021 Mar 11.
4
Giant Cellular Vacuoles Induced by Rare Earth Oxide Nanoparticles are Abnormally Enlarged Endo/Lysosomes and Promote mTOR-Dependent TFEB Nucleus Translocation.由稀土氧化物纳米颗粒诱导的巨大细胞空泡是异常增大的内体/溶酶体，并促进 mTOR 依赖性 TFEB 核转位。
Small. 2016 Nov;12(41):5759-5768. doi: 10.1002/smll.201601903. Epub 2016 Sep 4.
5
Multistep regulation of TFEB by MTORC1.MTORC1对转录因子EB（TFEB）的多步调控
Autophagy. 2017 Mar 4;13(3):464-472. doi: 10.1080/15548627.2016.1271514. Epub 2017 Jan 5.
6
New insights into desmosome regulation and pemphigus blistering as a desmosome-remodeling disease.深入了解桥粒调节和天疱疮水疱形成：一种桥粒重塑疾病。
Kaohsiung J Med Sci. 2013 Jan;29(1):1-13. doi: 10.1016/j.kjms.2012.08.001. Epub 2012 Oct 12.
7
Lysosomotropic drugs activate TFEB via lysosomal membrane fluidization and consequent inhibition of mTORC1 activity.溶酶体靶向药物通过溶酶体膜流化激活 TFEB，并由此抑制 mTORC1 活性。
Cell Death Dis. 2018 Dec 13;9(12):1191. doi: 10.1038/s41419-018-1227-0.
8
PEX5 regulates autophagy via the mTORC1-TFEB axis during starvation.PEX5 通过饥饿状态下的 mTORC1-TFEB 轴调节自噬。
Exp Mol Med. 2018 Apr 6;50(4):1-12. doi: 10.1038/s12276-017-0007-8.
9
The anti-tumor agent, Dp44mT, promotes nuclear translocation of TFEB via inhibition of the AMPK-mTORC1 axis.抗肿瘤剂 Dp44mT 通过抑制 AMPK-mTORC1 轴促进 TFEB 的核易位。
Biochim Biophys Acta Mol Basis Dis. 2020 Dec 1;1866(12):165970. doi: 10.1016/j.bbadis.2020.165970. Epub 2020 Sep 17.
10
Regulation of TFEB and V-ATPases by mTORC1.mTORC1 对 TFEB 和 V-ATPases 的调节。
EMBO J. 2011 Jul 29;30(16):3242-58. doi: 10.1038/emboj.2011.257.

本文引用的文献

1
ZnO nanoparticles impair autophagic flux and cell viability through the TRIM16-NRF2-p62 pathway in inflammatory keratinocytes.氧化锌纳米颗粒通过TRIM16-NRF2-p62途径损害炎症性角质形成细胞的自噬通量和细胞活力。
Food Chem Toxicol. 2023 Dec;182:114177. doi: 10.1016/j.fct.2023.114177. Epub 2023 Nov 15.
2
Epidermal stratification requires retromer-mediated desmoglein-1 recycling.表皮分层需要 retromer 介导的桥粒芯糖蛋白-1 循环。
Dev Cell. 2022 Dec 19;57(24):2683-2698.e8. doi: 10.1016/j.devcel.2022.11.010. Epub 2022 Dec 9.
3
How Nanoparticles Open the Paracellular Route of Biological Barriers: Mechanisms, Applications, and Prospects.
纳米颗粒如何打开生物屏障的细胞旁途径：机制、应用和前景。
ACS Nano. 2022 Oct 25;16(10):15627-15652. doi: 10.1021/acsnano.2c05317. Epub 2022 Sep 19.
4
Overview of Research into mTOR Inhibitors.mTOR 抑制剂研究概述。
Molecules. 2022 Aug 19;27(16):5295. doi: 10.3390/molecules27165295.
5
Tetrandrine Inhibits Skeletal Muscle Differentiation by Blocking Autophagic Flux.汉防己甲素通过阻断自噬通量抑制骨骼肌分化。
Int J Mol Sci. 2022 Jul 24;23(15):8148. doi: 10.3390/ijms23158148.
6
Past, present, and future perspectives of transcription factor EB (TFEB): mechanisms of regulation and association with disease.转录因子 EB（TFEB）的过去、现在和未来展望：调控机制及其与疾病的关联。
Cell Death Differ. 2022 Aug;29(8):1433-1449. doi: 10.1038/s41418-022-01028-6. Epub 2022 Jun 23.
7
Mechanisms Causing Acantholysis in Pemphigus-Lessons from Human Skin.棘层松解的发病机制在天疱疮中的研究——来自人类皮肤的启示。
Front Immunol. 2022 May 20;13:884067. doi: 10.3389/fimmu.2022.884067. eCollection 2022.
8
Zinc Oxide Nanoparticles: A Review on Its Applications in Dentistry.氧化锌纳米颗粒：牙科应用综述
Front Bioeng Biotechnol. 2022 May 19;10:917990. doi: 10.3389/fbioe.2022.917990. eCollection 2022.
9
Non-canonical mTORC1 signaling at the lysosome.溶酶体处的非典型mTORC1信号传导
Trends Cell Biol. 2022 Nov;32(11):920-931. doi: 10.1016/j.tcb.2022.04.012. Epub 2022 May 30.
10
Glutamine, MTOR and autophagy: a multiconnection relationship.谷氨酰胺、mTOR 和自噬：多连接关系。
Autophagy. 2022 Nov;18(11):2749-2750. doi: 10.1080/15548627.2022.2062875. Epub 2022 Apr 26.