Kjems J, Olesen S O, Garrett R A
Biochemistry. 1985 Jan 15;24(2):241-50. doi: 10.1021/bi00323a002.
The 5S RNAs from Bacillus stearothermophilus and Saccharomyces cerevisiae were probed by nucleotide-specific reagents, with a view to compare and contrast their higher order structures. The progressive unfolding of the RNAs during heating, in the presence and absence of magnesium, was monitored. Evidence was provided for the double-helical segments which occur in the secondary structural models of both RNAs. The results also placed constraints on the possible structuring of the remainder of the RNA and yielded some insight into ways of folding up the molecule. Together with the data from our earlier studies, employing ribonucleases, these results provide a detailed picture of the structuring and topography of the 5S RNAs. The main structural differences between the eubacterial and eukaryotic RNAs occur throughout the loop D/helix IV/loop E/helix V arm; in particular strong evidence is provided for loop D of the eukaryotic RNA being involved in a tertiary interaction.
用核苷酸特异性试剂探测嗜热脂肪芽孢杆菌和酿酒酵母的5S RNA，以比较和对比它们的高级结构。监测了在有镁和无镁情况下加热过程中RNA的逐步解折叠。为两种RNA二级结构模型中出现的双螺旋片段提供了证据。结果也对RNA其余部分的可能结构施加了限制，并对分子折叠方式有了一些了解。连同我们早期使用核糖核酸酶的研究数据，这些结果提供了5S RNA结构和拓扑的详细图景。真细菌和真核生物RNA之间的主要结构差异出现在整个环D/螺旋IV/环E/螺旋V臂；特别是为真核生物RNA的环D参与三级相互作用提供了有力证据。
