大肠杆菌5S RNA在50S核糖体亚基中的原位化学反应活性。

Chemical reactivity of E. coli 5S RNA in situ in the 50S ribosomal subunit.

作者信息

Silberklang M, RajBhandary U L, Lück A, Erdmann V A

出版信息

Nucleic Acids Res. 1983 Feb 11;11(3):605-17. doi: 10.1093/nar/11.3.605.

DOI:10.1093/nar/11.3.605

PMID:6340064

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325740/

Abstract

E. coli 50S ribosomal subunits were reacted with monoperphthalic acid under conditions in which non-base paired adenines are modified to their 1-N-oxides. 5S RNA was isolated from such chemically reacted subunits and the two modified adenines were identified as A73 and A99. The modified 5S RNA, when used in reconstitution of 50S subunits, yielded particles with reduced biological activity (50%). The results are discussed with respect to a recently proposed three-dimensional structure for 5S RNA, the interaction of the RNA with proteins E-L5, E-L18 and E-L25 and previously proposed interactions of 5S RNA with tRNA, 16S and 23S ribosomal RNAs.

摘要

在非碱基配对腺嘌呤被修饰为其1 - N -氧化物的条件下，使大肠杆菌50S核糖体亚基与单过氧邻苯二甲酸反应。从这种化学反应后的亚基中分离出5S RNA，并鉴定出两个被修饰的腺嘌呤为A73和A99。当将修饰后的5S RNA用于50S亚基的重组时，产生的颗粒生物活性降低（50%）。结合最近提出的5S RNA三维结构、RNA与蛋白质E - L5、E - L18和E - L25的相互作用以及先前提出的5S RNA与tRNA、16S和23S核糖体RNA的相互作用对结果进行了讨论。